Use flow cytometry for screening content ratings DNA in the nuclei of cells in the neuronal retina in rats
The paper presents the results of flow-cytometric study of DNA content in cells of neuronal retina intact Wistar rats. DNA content of the majority of retinal neuronal nuclei of rat retina was 2c (94,92 ± 0,94%). Also in the cells of the retina animals revealed the presence of particles tetraploid nuclei (DNA = 4c) - 4,98 ± 0,94% and the small percentage of nuclei containing DNA characteristic of S-phase - 0,10 ± 0,02%, that does not exclude the presence of Some synthetic activity of the DNA in the nuclei of the structure of the eye is normal. Background DNA fragmentation index (the percentage of fragmented DNA sites) was - 0,43 ± 0,11%. The absence of significant differences between the analytical performance of DNA histograms of cells in the retina of right and left eyes of animals, acceptable coefficients of variation peaks G0G1 and high accuracy in the application of this method to objectively assess the impact of screening drugs, various factors or factors on DNA content in cells of the retina: DNA fragmentation, DNA synthetic activity and ploidy.