The study of enzyme activity in the tear fluid and blood serum of rabbits in combined instillations of biopeloids and gentamicin in a bacterial keratitis
Introduction. Bacterial keratitis (BK) is characterized by an acute inflammatory reaction external structures of the anterior eye. The use of traditional antibiotic therapy does not always provide a positive therapeutic effect that is most frequently associated with the development of resistance to antibiotics, low immunity, dysbiosis, allergic reactions. The important points of pathogenetic therapy of BK are increased metabolic processes in the tissues of the anterior eye, improve microcirculation, activation mechanisms nonspecific defense. In this regard, very promising use of preparations containing natural BAS as effective pathogenic drugs of inflammatory diseases of the cornea. The study of enzyme activity in the tear fluid (TF) and serum of blood (SB) under the BK in the combined use of instillation biopeloidov and gentamicin allow to evaluate the effectiveness of the therapy, and predict the dynamics of the relief of the inflammatory process in the cornea.
Materials and methods. The research was conducted on 14 rabbits chinchilla weighing 2.5-3.0 kg, distributed into 2 groups: 1 - control (BK + 0.3% instillation solution and gentamicin); 2 - Experimental (BK + Combined biopeloidov instillation and 0.3% gentamicin solution). The TF and SB activity was determined enzymes lactate dehydrogenase (LDH), catalase, acid and alkaline phosphatase (AcP, AlP), lysozyme.
Results. It was found that in playing BK in TF rabbits changes the activity of enzymes: their performance in relation to the initial background accounted for LDH (+ 292%), alkaline phosphatase (60%), catalase (+ 522%), AcP (181 %), lysozyme (+162%), which is accompanied by a violation of the oxidation-reduction, antioxidant, membrane and antimicrobial processes in the cornea. The use of combined instillation biopeloidov and gentamicin in a BK has a normalizing effect, as evidenced by a lower level of activity of the enzymes LDH,AcP, AlP, catalase in the SB, and TF, increases the activity of lysozyme in TF.
Conclusions. Thus, the associated instillations of biopeloids and gentamicin in the treatment of bacterial keratitis have a normalizing effect on the enzyme activity, increase the activity of lysozyme in TF, reflecting the positive trend of faster relief of inflammation in the cornea.