Effect of 1-[4-(1-adamantyl)-phenoxy]-3-(n-benzene, n-dimethylamino) 2-propanol chloride on the candida albicans ultrastructure 3
Introduction. The spread of multidrug resistant strainscreated acritical need of the new effective antimicrobial agents. One of the promising chemical classes for its research and development are adamantane derivatives. After the screening studies compound KVM-97 was selected as the potent antifungal and antibacterial agent. In the present study, our purpose was to examine ultrastructural changes in the fungal cells under the influence of the compound KVM-97.
Materials and methods. CandidаalbicansNCTC 885/653 was used in all experiments.The minimum inhibitory concentration was determined by a macrodilution test in Saburo broth. Fungal cells were exposed to the 2.0 MIC of the KVM-97 for 1 h, 3 h, 6 h and 24 h. Ultrastructure of intact and treated C. albicans cells was examined by transmission electron microscopy after contrasting by osmium tetraoxide and lead citrate.
Results.The compound KVM-97 possesses a distinct antifungal activity against C. albicans, the minimum inhibitory concentration was 0.6 μg/ml. We found that the fungicidal concentration of compound caused ultrastructural changes of cytoplasm and mitochondria starting from 1 h of treatment. Progressive cytoplasmic deterioration, change in size and number of mitochondria, and loss of mitochondrial cristae were observed depending on duration of the treatment.
Conclusions. The detected alterations of C. albicans ultrastructure were considered to be specific response to the disorder in fungal cells energy process and apoptosis caused by KVM-97 treatment.