Imalepiphyseal cartilage and diaphysis of rats femoral bone during short-term prednisone application

  • A. V. Kalashnikov
  • A. T. Brusko
  • E. L. Kuziv
  • O. O. Viltsanyuk
  • L. I. Apuhovska
Keywords: osteoporosis, prednisolone, morphological changes in bone and cartilage during prednisone therapy


Introduction. Short-term appointment of glucocorticoids for treatmant various diseases is widely used in medicine, but the changes in bone and cartilage tissue, which are connected with such treatment, is studied insufficiently.

The aim of the study. Explore at the experiment morphological changes in bone and cartilage in the short-term administration of prednisolone.

Materials and methods. The study conducted on 34 linear Wistar species rats. Animals were divided into two series of experiments. The first series (17 rats), which was the control animals, are not treated with prednisolone. The second series of experiments animals (17 rats), were administered 0,5 mg prednisolone. On 30 day of the experiment, the animals were weighed and taken out of the experiment by an overdose of ether. Than for examination the materials were carried. In histological preparates was determined morphometrical indicators of proliferative processes activity in articular cartilage and epiphyseal cartilage, counted the number of common channels in the central vascular diaphysis for the determination of diaphysis remodeling bone during prednisolone administration. The data was conducted due to statistical analysis with subsequent comparison and determine their authenticity differences using T-Student test.

Research results. Studies have shown that the 30 days prednisolone introduction into rats, caused degenerative and necrotic changes in the articular and epiphyseal cartilage, compact and spongy bone epiphysis and metaphysis, accompanied by violation of their structure, resulting disorders formation of bone tissue and its plate structures, slowdown restructuring bone and bone growth in length and thickness. Results of osteometry were conducted by animal control series of experiments: rats femur length was 31,01 ± 0,05 mm and thickness - 3,49 ± 0,26 mm. While in a series of experiments where animals treated with prednisolone these figures were at the next level: the femur length was 28,81 ± 0,25 mm and the thickness was 3,07 ±04,0 mm, which was significantly (p <0, 05) lower than in the control group of animals and it was evidence of the negative effect of prednisone on these indicators. Research the number of central vascular channels at the site of the anterior histological sections of the middle third of the femoral diaphysis showed that in the control series of experiments the number was 20,43 ± 0,841 mm, while a series of experiments where animals administered prednisone their number was 37,43 ± 1,100 mm, which was significantly (p <0,05) higher than in the control series of experiments.

Conclusion. Results of morphological studies show that taking short-term courses of prednisolone caused degenerative and necrotic changes in articular epiphyseal cartilage and in a compact and spongy bone epiphysis and metaphysis. These changes were accompanied by a violation of their structure, results in the formation of bone disorders and its lamellar structures, slowdown restructuring of compact bone and lead to bone growth disorders in length and its thickness.

The obtained data indicate the need to develop methods for correcting these disorders, especially medication that would perform prophylaxis violation structural - functional organization, articular epiphyseal cartilage formation and reconstruction of bone tissue.

How to Cite
Kalashnikov, A. V., Brusko, A. T., Kuziv, E. L., Viltsanyuk, O. O., & Apuhovska, L. I. (2017). Imalepiphyseal cartilage and diaphysis of rats femoral bone during short-term prednisone application. Reports of Morphology, 21(2), 303-307. Retrieved from