Pathological changes in rats’ liver with chronic alcohol intoxication and after correction by quercetin and L-arginine L-glutamate depending on animals’ age


  • N. A. Rуkalo
  • L. А. Yarovenko
Keywords: liver, chronic alcoholic liver disease, fibrosis, morphological studies

Abstract

Introduction. During last years the amount of complications after chronic alcoholic liver disease has been increased various etiologies, first of all – alcohol. Diagnostic techniques of liver pathologies include both clinical and morphological methods, which allow assessing of spare liver capacity.

Current research is aimed at improving the pathogenetic therapy. The most accurate measure of efficiency is the morphological study. The main aim is to learn age features of pathomorphological changes of rats liver tissue with chronic alcoholic liver intoxication and their possible correction by quercetin and L-arginine-L-glutamate.

Materials and methods. The study was conducted on 120 white female rats of three age groups: immature animals (1.5 month; starting weight – 60-80 g.), mature young (6 months, starting weight – 180-220 g.); old animals (20 months, starting weight – 300-320 g.). Each group of animals was devided into four groups such as 10 animals in each: intact animals; animals with chronic alcoholic liver disease (daily administered 14-18 g / kg of ethanol for 12 weeks); animals with chronic alcoholic liver disease with the correction using quercetin (100 mg/kg); animals with chronic alcoholic liver disease with the correction using L-arginine-L-glutamate (35mg/kg). The duration of administration of 12 weeks.

Rats were taken out of the experiment simultaneously using decapitation method under ether narcosis. The liver was removed, which was fixed using 10% neutral formalin solution, conducted through the spirits battery of rising concentrations and steeped in paraffin. During histological study were used microtome sections (5 microns), which were stained by hematoxylin and eosin and Van Gieson's stain. Histological microspecimens were studied by light optical microscope “Olimpus BN-2” with enlarging from 100 up to 400 times and digital images were gained.

Results: Established that under conditions of experimental chronic alcoholic intoxication in rats of different age develop liver damage, which is based on necrotic and dystrophic changes with prevalence of fat and protein intracellular degeneration. Histological changes in rats of all age groups accompanied by violation of lobular structure of liver parenchyma. The central veins and sinusoids, beamed cell organization can’t be visualized. Hepatocytes have a severe level of pulverulent fat and hydropic protein degenerations, some of them were deformed with fuzzy contours. The vessels of portal tracts have been totally expanded, and their gap were filled by erythrocytes, there was marked perivascular edema, which was combined with lymphoma and histiocytic infiltration. It should be noted that hepatocytes destruction caused by pulverulent fat and intracellular protein degenerations have more locally character in the old animals in comparence with the immature and mature young animals. After pathogenetic correction by quercetin and L-arginine-L-glutamate was observed next morphological changes, which in some cases were characterized by lower intensity of necrotic and dystrophic change and a decrease in collagen fibers, which confirm the positive impact of the selected remedy. The obtained results show that L-arginine-L-glutamate has more hepatoprotective effect in rats with chronic alcoholic liver disease, compared with quercetin.

Conclusions:

  1. Established that in rats of different age groups on the background of chronic alcoholic liver disease develop liver damage which is expressed by necrotic and dystrophic changes with prevalence of fat and protein intracellular degeneration, morphologically. According to the same experimental conditions, more vulnerable to the toxic ethanol effect were immature and old animals, because morphofunctional changes of liver structure characterized by more local dystrophic hepatocytes’ changes in comparence to the young immature animals.
  2. Against the backdrop of the introduction of ethanol in the liver of rats of all ages observed the proliferation of collagen fibers with the development of fibrosis. Proved that quercetin and L-arginine-L-glutamate under conditions HAUP show antyfibrotychnyy effect.
  1. Histological study shows that hepatoprotector L-arginine-L-glutamate contributes to more effective and intensive recovery of structural liver components during chronic alcoholic liver disease, compared to quercetin, as evidenced by significant reduction of necrotic symptoms, reduction of manifestation of fat and protein intracellular liver degeneration, reduction of fibrosis and acceleration of recovery process of hepatocytes.
Published
2017-10-27
How to Cite
RуkaloN. A., & YarovenkoL. А. (2017). Pathological changes in rats’ liver with chronic alcohol intoxication and after correction by quercetin and L-arginine L-glutamate depending on animals’ age. Reports of Morphology, 21(2), 308-312. Retrieved from https://morphology-journal.com/index.php/journal/article/view/156
Section
ORIGINAL INVESTIGATIONS