Peculiarities of structural changes of pancreas in conditions of experimental atherosclerosis correction
Introduction. Atherosclerosis and its consequences and, above all, cardiovascular diseases and diabetes hold the first positions among the most widespread reasons for disability and mortality of adult population. In this light, pancreas state is a matter of interest, particularly, taking into account the effect of some medicines used in atherosclerosis and its consequences. Objectives of this research is to study the peculiarities of structural changes of pancreas in conditions of experimental atherosclerosis correction with the help of medicines of different mechanisms of effect and of different origin. The goal of this research included performing of histologic and morphometric assessment of structural changes of cellular elements of pancreas endocrine part in conditions of experiment and comparing of obtained results of effects from medicines.
Materials and methods. Experimental atherosclerosis of rabbits was caused by the classical Anichkov’s method within three months. During the fourth month the rabbits of three groups were given the medicines: Vinboron (spasmolysant) – a national synthetical preparation of benzofuran line, Polysorbate (atoxyl) – enterosorbent of siliceous nature and Polysponinum – a plant preparation of hypocholesteremic action. For morphometric study pancreas of all experimental groups and intact rabbits (taken for comparison) was taken. After fixation in Bouin liquid the paraffin sections were coloured by Dyban’s method.
Results. In experimental atherosclerosis without correction in the most pancreatic islets of rabbits the vascular disorders can be defined and the changes of insular apparatus are developed that can be characterized by size reduction of islet cells and their nucleuses (33,72±0,91 micrometers3 against 89,89±3,63 in intact animals) as well as the reduction level of aldehyde-fuchsinophil granulosis in cytoplasm. Animals that were given Vinboron, Polysorbate and Polysponinum had the increased level of aldehyde-fuchsinophil granulosis and nucleus volume up to 45,96±3,12, 50,57±0,45, 50,91±0,68 micrometers3 correspondingly in comparison with those who were untreated. Functional index that is B-cells to A-cells ratio does not change and is 4:1.
Conclusions. 1. In experimental atherosclerosis the signs of hypofunction of insular apparatus of pancreas can be defined. 2. In conditions of taking Vinboron, Polysorbate and Polysponinum the signs of hypofunction reduce.