Ultrastructual changes in brain cells of rats under intoxication 1, 2-dichloroethane


  • L. V. Yanitska
Keywords: ultrastructural changes, brain, 1,2-dichloroethane, xenobiotics, rats

Abstract

Introduction. 1,2-dichloroethane, arriving in humans or warm-blooded animals, causing damage to membrane structures of many organs and tissues, including liver, myocardium, brain, kidneys, resulting in severe nekrodystrofical of these lesions [Hubskyy, 2001; Luzhnykov, 2012].

Studies have been conducted morphological cellular structures of the brain in acute poisoning 1,2-dichloroethane and after correction nicotinamide.

Materials and Methods. Ultrastructural changes of brain studied by electron microscopy. For electron microscopic studies of liver tissue were fixed in 2% solution OSO4, for to fill objects used araldyt. Ultrathin sections were analyzed in electron microscopes UEMV - 100 B and EMR - 100 L.

Results. Electron microscope examination of the cerebral hemispheres in terms of 1,2-dyhloretanovoyi intoxication showed that distinct ultrastructural changes observed in all structural components of the cerebral cortex. In neurons observed mosaic structure. There are cells that contain a nucleus with a few enlightened kariopolazmoyu unchanged and organelles biloksyntetychnoho energy plan and be placed in the cytosol of moderate density.

Neurons are marked with tightened cytoplasm and karioplazmoyu. They usually altered in form - as the cells themselves, and their nucleus. For a typical nuclear chromatin is the formation of a large number brylok across karioplazmi. Compartments endoplasmic reticulum dramatically compacted, contain small amounts of ribosomes and shifted to the periphery of the cell.

Tubules diktiosom Golgi complex and advanced dekompleksovani. In the mitochondria appear to change destructive Christie and plots lysis matrix, which often reach large sizes, sometimes almost remains only the external mitochondrial membrane.

In neuropile damage primarily related synaptic connections, which number reduced, and their presynaptic terminal by changing "light" type, that is swelling of the past and the disappearance of synaptic vesicles. In aksoplazmi neyrofilamenty are separate and mostly degenerative altered mitochondria.

In space there is a distinct perivascular edema and accumulation of lipid droplets. The lumen of blood microvessels filled plazmykrovi protein precipitate and separate erythrocytes. Sladzhfenomenu not clearly marked. There is a lack of endothelial cells and pericytes elektronnouschilnenyh and better preservation of ultrastructure of endothelial cells. Also observed thinning endothelial hemomikrosudyn will send to the thickness of the plasma membrane, areas of local lysis plasma membrane and cytoplasm of endothelial significant thickening of the basement membrane.

When you enter nicotinamide conditions for 1, 2-dichloroethane poisoning decrease majority of pathological changes in the structural components of the cerebral cortex.

Conclusions. 1. Ultrastructural changes in brain cells of rats under intoxication hloralkanamy characterized by morphological manifestations of hypoxia histotoksychnoyi neurons and glial cells, in particular 1,2-dichloroethane active phenomena in the formation and synaptic mitochondria, microcirculation disturbance in the bloodstream. 2. Correction nicotinamide led to less pronounced ultrastructural changes in the brain tissue of rats poisoned 1,2-dichloroethane.

Published
2017-10-27
How to Cite
Yanitska, L. V. (2017). Ultrastructual changes in brain cells of rats under intoxication 1, 2-dichloroethane. Reports of Morphology, 21(2), 324-327. Retrieved from https://morphology-journal.com/index.php/journal/article/view/160
Section
ORIGINAL INVESTIGATIONS