Influence of hyperhomocysteinemia on nonalcoholic fatty liver disease formation in rats
To study the effect of hyperhomocysteinemia on the development of non-alcoholic fatty liver disease in rats were examined a number of biochemical parameters of blood serum and liver homogenate of 40 rats. Nonalcoholic fatty liver disease in chronic hyperhomocysteinemia leads to the progression of oxidative stress in the liver tissues of rats. This is manifested by increased NADPH- oxidase activity, protein amount of carbonyl groups in liver homogenate and malondialdehyde concentration in liver homogenate and serum. This reduces the activity of antioxidant enzymes, such as thioredoxin reductase, glutathione peroxidase and superoxide dismutase in liver homogenate. Hyperhomocysteinemia in rats kept on high-fat diet leads to a significant increase in the number of hepatocytes with cytoplasmic steatosis atomized. These data confirm that homocysteine is a factor in the progression of nonalcoholic fatty liver disease.