ELECTRON MICROSCOPIC DIAGNOSTICS OF APOPTOSIS PROCESSES UNDER SIMULATION CONDITIONS IN THE EXPERIMENT OF ACUTE PYELONEPHRITIS AND CONCOMITANT DIABETES MELLITUS TYPE I AND II


  • S.O. Borysov Odessa National Medical University, Department of Urology and Nephrology, Odessa, Ukraine
  • F.I. Kostyev Odessa National Medical University, Department of Urology and Nephrology, Odessa, Ukraine
  • O.V. Borysov Odessa National Medical University, Department of Urology and Nephrology, Odessa, Ukraine
  • N.I. Molchanyuk Odessa National Medical University, Department of Urology and Nephrology, Odessa, Ukraine
Keywords: modeling, pathogenesis, pyelonephritis, diabetes mellitus, electron microscopy, cortical and cerebral matter, apoptosis

Abstract

According to literature data, in the conditions of the balance of pro- and anti-inflammatory factors, the development of the infectious-inflammatory process tends to progress and more severe with subsequent transformation into chronic inflammation with corresponding morphofunctional effects. Under such critical conditions, an apoptosis is likely to play a crucial protective role, which can eliminate the excessive accumulation of aggressive inflammatory effects and effectively eliminate them, which in the future prevents the probable transformation into a persistent form. The aim of the work was to study the features of the early dynamic processes of apoptosis in the tissues of the kidneys under simulation conditions in the experiment of acute pyelonephritis and concomitant diabetes mellitus type I and II. The purpose of the study was to study the features of the early dynamic processes of apoptosis in the tissues of the kidneys under simulation conditions in the experiment of acute pyelonephritis and concomitant type I and type II diabetes. The work was performed on 300 adult Wistar rats, which were divided into 4 groups. Fragments of animal’s kidneys were studied and photographed in an electron microscope PEM-100-01. The results showed that after modeling in the animals of pyelonephritis in the nephrons there were no significant ultrastructural changes. The structure of the podocytes of the outer sheet was almost the same as the structure of the podocytes of the control material, and in some cells there were signs of activation of their metabolic activity. In the structure of the podocytes of the inner leaf of the capsule, dystrophic changes of the internal membrane of the mitochondria were established. In kidney medulla, the structure of the glomerulus was more preserved than in the cortical. Pathological changes of the proximal and distal tubular podocytes, as well as interstitial tissue, are more pronounced than the renal glomeruli. Under the modeling of the common model of pyelonephritis and type 1 diabetes, more pronounced morphological changes occur: destructive changes in the endothelial cells of the glomerular capillaries, the homogenization of the structure of the basement membrane occurs and the mesangial tissue is significantly enlarged. In nephrons and tubules of cortex, changes are manifested to a much greater extent than in the kidney medulla. When studying in clinical conditions the pathogenetic features of acute pyelonephritis in conditions of concomitant diabetes mellitus it is expedient to carry out electron microscopic research with the aim of choosing the optimal corrective therapeutic effect and preventing the unfavorable course of infectious and inflammatory process and its transformation into persistent form. EM (electron-microscopic) studies are highly informative in the study of pathological changes and early dynamic processes of apoptosis in renal tissues in the design of acute pyelonephritis and concomitant diabetes mellitus I and II in the experimental conditions.

References

[1] Dorogoj, A. P. (2007). Life expectancy, potential labor potential losses, and death in diabetes mellitus. International Endocrinology Journal, 3(9), 10–12.

[2] Kuznetsova, O. P., Vorobiev, P. A., & Yakovlev, S. V. (1997). Urinary tract infections. Russian Medical Journal, 1, 4–13.

[3] Novikov, V. S. (1996). Programmed cell death. S.-Pb.: Science. Retrieved from humbio.ru/humbio/starenie/0001d36e.htm

[4] Pak, L. B., Dubikov, A. I., Kabantseva, T. A., Vasilyuk, A. A., & Grigoryan, O. M. (2013). Apoptosis and kidney pathology. Nephrology, 17(4), 36-43. ISSN 1561-6274.

[5] Popov, S. V., Guseinov, R. G., Gorshkov, A. N., Sivak, K. V., Yablonskiy, P. K., Skryabin, O. N., & Vinogradova T. I. (2016). Changes in the ultrastructural organization of the kidney under the conditions of experimentally modeled thermal ischemia in surgical intervention. Bulletin of St. Petersburg University, 11(1), 104-119. Retrieved from vestnik.spbu.ru/html16/s11/s11v1/10.

[6] Tomilina, N. A., & Bikbov, B. T. (2005). Epidemiology of chronic renal failure and new approaches to the classification and evaluation of the severity of chronic progressive kidney disease. Therapeutic archive, 6, 87-89. Retrieved from onco.tnimc.ru/journal/lib-archive/terapevticheskiy-arkhiv/

[7] Vinnichenko, L. N. (1980). Comparative ultrastructure of nephron. L.: Nauka, 136. Retrieved from www.cytspb.rssi.ru/books/vinnichenko_ru.htm

[8] Becker, F., Van Poppel, H., Hakenberg, O. W., Stief, C., Gill, I., Guazzoni, G., … Stöckle, M. (2009). Assessing the impact of ischaemia time during partial nephrectomy. Eur. Urol., 56, 625 – 635. doi: 10.1016/j.eururo.2009.07.016.

[9] Bhayani, S. B., Rha, K. H., Pinto, P. A., Ong, A. M., Allaf, M. E., Trock, B. J. … Kavoussi, L. R. (2004). Laparoscopic partial nephrectomy: effect of warm ischemia on serum creatinine. J. Urol., 172(4), 1, 1264–1266. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/15371820

[10] Donohoe, J. F., Venkatachalam, M. A., Bernard, D. B. & Levinsky, N. G. (1978). Tubular leakage and obstruction after renal ischemia: structural-functional correlations. Kidney Int., 13(3), 208–222. https://doi.org/10.1038/ki.1978.31

[11] Gill, I. S., Colombo, J. R. Jr., Moinzadeh, A., Finelli A., Ukimura O., Tucker K., … Desai, M. (2006). Laparoscopic partial nephrectomy in solitary kidney. J. Urol., 175(2), 454–458. DOI: 10.1016/S0022-5347(05)00150-3

[12] Hansson, J., Hultenby, K., Cramnert, C., Pontén, F., Jansson, H., Lindgren, D. … Johansson, M. E. (2014). Evidence for a morphologically distinct and functionally robust cell type in the proximal tubules of human kidney. Hum. Pathol., 45, 382–393. DOI: 10.1016/j.humpath.2013.10.003

[13] Humphreys, B. D., Czerniak, S., Dirocco, D. P., Hasnain, W., Cheema, R., & Bonventre, J. V. (2011). Repair of injured proximal tubule does not involve specialized progenitors. Proc. Natl. Acad. Sci. USA, 108(22), 9226–9231. doi: 10.1073/pnas.1100629108.

[14] Porpiglia, F., Renard, J., Billia, M., Musso, F., Volpe, A, Burruni, R. … Scarpa, R. M. (2007). Is renal warm ischemia over 30 minutes during laparoscopic partial nephrectomy possible? One-year results of a prospective study. Eur. Urol., 52(4), 1170–1178. DOI: 10.1016/j.eururo.2007.04.024

[15] Reуnoldes, E. S. (1963). The use of lead citrate at higt pH an electronopaque stain in electron microscopy. J. of Cell Biology, 17, 208–212. Retrieved from https://www.ncbi.nlm.nih.gov/pubmed/13986422

[16] Thompson, R. H., Lane, B. R., Lohse, C. M., Leibovich, B. C., Fergany, A., Frank, I. … Campbell, S. C. (2010). Every minute counts when the renal hilum is clamped during partial nephrectomy. Eur. Urol., 2010, 58(3), 340–345. doi: 10.1016/j.eururo.2010.05.047

[17] Tirapelli, L. F., Barione, D. F., Trazzi, B. F. M., Tirapelli, D. P. C., Novas, P. C., Silva, C. S. … Martins, A. C. P. (2009). Comparison of two models for evaluation histopathology of experimental renal ischemia. Transplant. Proc., 41, 4083–4087.

[18] Verlander, J. W. (1998). Normal ultrastructure of the kidney and lower urinary tract. Toxicol. Pathol., 26(1), 1–17. DOI: 10.1177/019262339802600101
Published
2018-03-29
How to Cite
Borysov, S., Kostyev, F., Borysov, O., & Molchanyuk, N. (2018). ELECTRON MICROSCOPIC DIAGNOSTICS OF APOPTOSIS PROCESSES UNDER SIMULATION CONDITIONS IN THE EXPERIMENT OF ACUTE PYELONEPHRITIS AND CONCOMITANT DIABETES MELLITUS TYPE I AND II. Reports of Morphology, 24(1), 39-46. https://doi.org/https://doi.org/10.31393/morphology-journal-2018-24(1)-07