Histochemical picture in the skin of rats 1, 3, 7, 14, 21 and 30 days after burning of ІІ-ІІІ degrees on the background of injection during first 7 days of 0.9% NaCl solution

  • О.Ye. Маievskyi National Pirogov Memorial Medical University, Vinnytsya, Ukraine
  • К.S. Volkov SHEE “Ternopil Ivan Horbachevsky State Medical University of the Ministry of Healthcare of Ukraine”, Ternopil, Ukraine
  • Z.M. Nebesna SHEE “Ternopil Ivan Horbachevsky State Medical University of the Ministry of Healthcare of Ukraine”, Ternopil, Ukraine
  • Ye.V. Mironov National Pirogov Memorial Medical University, Vinnytsya, Ukraine
  • A.V. Shayuk Zhytomyr Ivan Franko State University, Zhytomyr, Ukraine
Keywords: thermal burns of the skin, rats, histochemical studies


For pathogenetically grounded therapy of burn disease, aimed at reducing the severity of the pathological process, the rapid recovery of microcirculation and stimulation of reparative processes, it is necessary to study the fine mechanisms of reparative regeneration in the skin on the microscopic and biochemical levels. The purpose of the study is to investigate the state of elastic and collagen fibers, as well as glycoproteins and glycosaminoglycans in the intercellular substance of the dermis of rats 1, 3, 7, 14, 21 and 30 days after the burning of the II-III degree, with the background of the injection during first 7 days of 0.9% NaCl solution. An experimental study of structural changes in the skin after burn injury was performed on laboratory white rats, males weighing 150-160 g. Rats were divided into 2 groups, which previously, under the conditions of propofol anesthesia 60 mg/kg internally, catheterization of the femoral vein and depilation lateral surfaces of the trunk of rats. Group 1 - animals without thermal trauma of the skin, which once a day for the first 7 days were injected intravenous infusion of 0.9% NaCl solution in a dose of 10 ml per kg. Group 2 - animals who were also injected once a day with the first 7 days of infusion of 0.9% NaCl solution in a dose of 10 ml per kg after 2-3 degree skin burns, with a total area of 21-23% of the body surface of the rat. Euthanasia of rats was performed after propofol anesthesia (60 mg/kg i/v) by decapitation. Changes in the histochemical structure of bits of skin from the edge of the wound were studied after 1, 3, 7, 14, 21, and 30 days from the beginning of the experiment. Histological sections 5-6 microns thick were stained with Weigert’s elastic stain and dyed with picric acid by Van Gieson’s method (for the detection of elastic and collagen fibers); the PAS + Hale reaction was performed by the Moury method (detection of glycoproteins and glycosaminoglycans in the intercellular substance of the dermis). The histochemical investigations of the area of the II-III degree of skin damaged by burns on the background of the injection of 0.9% NaCl solution found that the degree of change depends on the duration of the experiment. In the early stages of the experiment, the reorganization of the intercellular substance of the dermis manifests itself as damage to the fibrous structures of its papillary and reticular layers. Histochemically, the components of amorphous connective tissue substance are dominated by glycoproteins, the content of acidic glycosaminoglycans is small. In the late stages of the experiment (after 14, 21 and 30 days) in the connective tissue of the affected skin in the central and marginal regions poorly structured collagen and elastic fibers are observed. The pronounced “Hale”-positive coloration reflects an increase in the content of sulfated glycosaminoglycans in the amorphous substance of the connective tissue. Thus, the experimental thermal trauma of skin II-III degree on the background of the introduction during the first 7 days, 0.9% of the NaCl solution is histochemically characterized by reorganization of the intercellular substance of the connective tissue of the dermis. In the early stages of the experiment there is damage to the fibrous structures of the papillary and reticular layers, the prevalence of glycoproteins and the insignificant content of acidic glycosaminoglycans in the amorphous substance, indicating a significant inhibition of adaptive-compensatory processes. In the long term, after the thermal defeat in the dermis, histochemically, an increase in the amount of acid glycosaminoglycans is detected and the PAS positivity of the collagen fibers of the intercellular substance is well expressed. This indicates a slow updating of the amorphous substance and fibrous structures of the connective tissue of the dermis, a violation of regenerative processes.


[1] Bikova, I. Yu., Yefimenko, N. A., & Gumanenko, Ye. К. (2009). Field Surgery: national guide. М.: GEOTAR - Media.
[2] Breitkreutz, D., Mirancea, N., & Nischt, R. (2009). Basement membranes in skin: unique matrix structures with diverse functions? Histochem. Cell. Biol., 132(1), 1-10. doi: 0.1007/s00418-009-0586-0
[3] Chaikovskii, Yu. В., Korol, А. Р., & Makarovа., О. І. (2014). Ultrastructural features of respiratory tract lungs of rats in remote period after thermal burn its skin. Biomedical and Biosocial Anthropology, 23, 57-62.
[4] Chaikovskii, Yu. В., Makarovа., О. І., Chereshniuk, I. L., & Lysenko, D. A. (2014). Comparative characteristics of cell cycle and DNA fragmentation of lung cells in rats after skin burn injury. World of Medicine and Biology, 2(44), 181-185.
[5] Delavary, B. M., van der Veer, W. M., van Egmond, M., Niessen, F. B., & Beelen, R. H. J. (2011). Macrophages in skin injury and repair. Immunobiology, 216(7), 753-762. doi: 10.1016/j.imbio.2011.01.001
[6] Fatemi, M. J., Momeni, M., Tavakoli, A., Bagheri, T., Hosseini, A., Araghi, S., … Zavareh, A. (2018). Treatment of third-degree burn wounds in animal specimens: acellular dermis or partial-thickness skin graft. Ann. Burns Fire Disasters, 31(2), 144-148. PMID: 30374268
[7] Galunko, G. М. (2017). Histological changes in the small intestine in the advanced stages of burn disease. World of Medicine and Biology, 3(61), 90-96. doi: 10.26724 / 2079-8334-2017-3-61-90-96
[8] Gavryluk, А. О., Gunas, I. V., Galunko, G. М., Chereshniuk, І. L., & Lysenko, D. A. (2017). Indicators of the cell cycle and fragmentation of DNA of cells of small intestinal mucosa through 14, 21 and 30 days after burn skin damage on the background of infusion of 0,9% NaCl solution. Biomedical and Biosocial Аnthropology, 29, 104-108.
[9] Goralskiy, L. P., Homich, V. Т., & Kononskiy, О. І. (2011). Fundamentals of histological technique and morphofunctional methods of research in norm and in pathology. Zhitomir: Polissya.
[10] Grin, І. V., Zvyaginceva, Т. V., Naumova, О. V., & Grin, V. V. (2015). Influence of tiotriazolin ointment with nanoparticles of silver on morphological changes of skin of rats after thermal burn. Clinical Pharmacy, Pharmacotherapy and Medical Standardization, 3-4(27-28), 126-130.
[11] Gunas, I. V., Guminskiy, Yu. I., Ocheretna, N. P., Lysenko, D. A., Kovalchuk, О. І., Dzevulska, І. V., & Cherkasov, E. V. (2018). Indicators cell cycle and dna fragmentation of spleen cells in early terms after thermal burns of skin at the background of introduction 0.9% NaCl solution. World of Medicine and Biology, 1(63), 116-120. doi: 10.26.724/2079-8334-2018-1-63-116-120
[12] Gunas, I. V., Kondratskiy, B. О., Nurmetova, І. К., Dzevulska, І. V., Kovalchuk, О. І., Cherkasov, Ye. V., … Sitnik, О. V. (2012). Dynamics of changes in the level of endogenous intoxication in rats organisms for a month after the skin burn of II-III degree, 21-23% of the body surface area and its correction by infusion solutions of Laсtoprotein with sorbitol and HAES-LX-5%. Ukrainian morphological almanac, 10(4), 29-34.
[13] Gunas, I. V., Ocheretnyuk, А. О., Chereshniuk, І. L., & Lysenko, D. A. (2013). Features of cell cycle and lung cell DNA fragmentation in rats within 1, 3 and 7 days after skin burns. Clinical and experimental morphology, 3(7), 38-43.
[14] Gunas, I., Dovgan, I., & Masur, O. (1997). Method of thermal burn trauma correction by means of cryoinfluence. Abstracts are presented in zusammen mit der Polish Anatomical Society with the participation of the Association des Anatomistes Verhandlungen der Anatomischen Gesellschaft, Olsztyn (р. 105). Jena – München: Der Urban & Fischer Verlag.
[15] Gurtner, G. C., Werner, S., Barrandon, Y., & Longaker, M. T. (2008). Wound repair and regeneration. J. Nature, 453, 314-321. doi: 10.1038/nature07039
[16] Hoganson, D. M., O’Doherty, E. M., Owens, G. E., Harilal, D. O., Goldman, S. M., Bowley, C. M., … Vacanti, J. P. (2010). The retention of extracellular matrix proteins and angiogenic and mitogenic cytokines in a decellularized porcine dermis. Biomaterials, 31(26), 6730-6737. doi: 10.1016/j.biomaterials.2010.05.019
[17] Imasheva, А. К., & Lazko, М. V. (2009). Features of the regenerative processes of the skin during thermal burns. Basic research, 5, 22-24.
[18] Kallinen, O., Maisniemi, K., Böhling, T., Tukiainen, E., & Koljonen, V. (2012). Multiple organ failure as a cause of death in patients with severe burns. J. Burn Care Res, 33(2), 206-211. doi: 10.1097/BCR.0b013e3182331e73
[19] Kearney, L., Francis, E. C., & Clover, A. J. (2018). New technologies in global burn care – a review of recent advances. Int. J. Burns Trauma, 8(4), 77-87. PMID: 30245912
[20] Kozinets, G. P., Cigankov, V. P., & Kovalenko, О. М. (2012). Modern infusion therapy in burn shock. Ukrainian Journal of Hematology and Transfusiology, 4, 39-42.
[21] Kraft, R., Herndon, D. N., Al-Mousawi, A. M., Williams, F. N., Finnerty, C. C., & Jeschke, M. G. (2013). Burn size and survival probability in pediatric patients in modern burn care. Lancet, 379(9820), 1013-1021. doi: 10.1016/S0140-6736(11)61345-7
[22] Kramar, S. B., & Volkov, К. S. (2014). Histologic and histochemical changes of the damaged area of the skin in the dynamics after the experimental thermal trauma. Journal of Medical and Biological Problems, 4(46), 182-185.
[23] Lachtadyr, T. V. (2017). Structural changes of rat’s renal cortex in late period of skin burn injury under the conditions of the infusion by isotonic Natrium cloridum solution. Reports of Morphology, 23(2), 211-218.
[24] Lataillade, J., Bey, E., Thepenier, C., Prat, M., Leclerc, T., & Bargues, L. (2010). Skin engineering for burns. Bull. Acad. Nat. Med., 194(7), 1339- 1351. PMID: 22043629
[25] Lee, M. O. (1989). Determination of the surface area of the white rat with its application to the expression of metabolic results. Am. J. Physiol., 24, 1223. https://doi.org/10.1152/ajplegacy.1929.89.1.24
[26] Musina, L. А., & Nurayeva, А. B. (2015). Healing of burn wounds with the use of allogeneic biomaterials (experimental morphological study). Bulletin of the Orenburg State University, 12(187), 142-145.
[27] Pereira, R. F., Barrias, C. C., Granja, P. L., & Bartolo, P. J. (2013). Advanced biofabrication strategies for skin regeneration and repair. Nanomedicine (Lond), 8(4), 603-621. doi: 10.2217/nnm.13.50
[28] Regas, F. C., & Ehrlich, H. P. (1992). Elucidating the vascular response to burns with a new rat model. J. Trauma, 32, 5, 557-563. PMID: 1588642
[29] Rnjak, J., Weiss, S. G., Mithieux, S. M., & Weiss A. S. (2011). Severe burn injuries and the role of elastin in the design of dermal substitutes. Tissue Engineering Part B Reviews, 17(2), 81-91. doi: 10.1089/ten.TEB.2010.0452
[30] Sarkisov, D. S., & Perova, Yu. L. (1996). Microscopic technique. М.: Medicine.
[31] Schаnо, V. P., Grin, V. К., Fistal, E. Ya., Miminoshvili, О.I., Zayats, Yu.V., & Chorniy, V. I. (2006). Burn shock. Donetsk: Southeast.
[32] Shapovalov, V. М., & Gladkov, R. V. (2014). Peacetime blast damage: epidemiology, pathogenesis and major clinical manifestations. Biomedical and socio-psychological problems of safety in the extreme situations, 3, 5-16.
[33] Shi, H., Weng, T., Han, C., & Wang, X. (2018). Improved Dermal Regeneration Using a Combination of Dermal Substitutes and Dermal Fibroblast Optimization: A Hypothesis. Med. Sci. Monit., 24, 5457-5461. doi: 10.12659/MSM.909743
[34] Swanson, J. W., Otto, A. M., Gibran, N. S., Klein, M. B., Kramer, C. B., Heimbach, D. M., & Pham, T. N. (2013). Trajectories to death in patients with burn injury. J. Trauma Acute Care Surg., 74(1), 282-288. doi: 10.1097/TA.0b013e3182788a1c
[35] van der Veen, V. C., van der Wal, M. B., van Leeuwen, M. C., Ulrich, M. M., & Middelkoop, E. (2010). Biological background of dermal substitutes. Burns, 36(3), 305-321. doi: 10.1016/j.burns.2009.07.012
[36] Voigt, J., & Driver, V. R. (2012). Hyaluronic acid derivatives and their healing effect on burns, epithelial surgical wounds, and chronic wounds: a systematic review and metaanalysis of randomized controlled trials. Wound Repair Regen, 20(3), 317-331. doi: 10.1111/j.1524-475X.2012.00777.x
[37] Woodley, D. T. (2017). Distinct Fibroblasts in the Papillary and Reticular Dermis: Implications for Wound Healing. Dermatol. Clin., 35(1), 95-100. doi: 10.1016/j.det.2016.07.004
How to Cite
МаievskyiО., VolkovК., Nebesna, Z., Mironov, Y., & Shayuk, A. (2018). Histochemical picture in the skin of rats 1, 3, 7, 14, 21 and 30 days after burning of ІІ-ІІІ degrees on the background of injection during first 7 days of 0.9% NaCl solution. Reports of Morphology, 24(2), 50-56. https://doi.org/10.31393/morphology-journal-2018-24(2)-08

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