Features of structural-morphological changes in cases of experimental intestinal antibiotic-induced dysbiosis
Nowadays, scientists often define dysbiosis as a condition of a microbial ecological system, in which there is a simultaneous abnormality of the functions and interaction mechanisms of its key components: macroorganism and indigenous microbiota associated with the mucous membranes of cavities and skin. At the same time, obviously, the basis of all these processes is changes of structural intestinal components that are caused by qualitative and quantitative changes in the normal microflora. Purpose: to study the ultrastructural organization of the mucous membrane of the small intestine of mice after the formation of dysbiosis of the intestine. Outbred white mice in the number of 40 units (20 - experimental and 20 control) was served as an experimental model. Antibacterial drugs (ampicillin, metronidazole and gentamicin) are used to form dysbiosis. The conducted experiments are allowed to establish that the using of antibacterial drugs in the above-mentioned doses contributes to shortening the length of the microvillus and their reduction (disappearance) in some places, destruction with subsequent disintegration. According to the results of electronograms analysis, the assumption was made about stimulating the secretory function of the small intestine enterocytes by powerful doses of antibacterial drugs. In addition, it was found that the formation of dysbiotic disorders is accompanied by a defect of the connection between epithelial cells due to the expansion of the intercellular space and the disappearance of the dense plate. Research results also indicate that antibiotics that were used in the experiment can cause development of apoptosis. In addition, it has been shown that, on the background of the dysbiotic disorders formation, the activation of immune processes is taking place, as evidenced by the appearance of a significant number of Paneth cells, plasma cells with enlarged tubules, apparently due to their filling with immunoglobulins, as well as the growth of numbers of luminalis eosinophils and basophils. The ability of antibiotics to form dysbiotic states with pronounced cytodestructive disorders in the epithelium of the small intestine with the development of apoptosis was substantiated; the argument about the immune stimulating effect of antibiotic induced dysbiosis is argued.
 Bilen, M., Dufour, J. C., Lagier, J. C., Cadoret, F., Daoud, Z., Dubourg, G., & Raoult, D. (2018). The contribution of culturomics to the repertoire of isolated human bacterial and archaeal species. Microbiome, 6(1), 94. doi: 10.1186/s40168-018-0485-5
 Bobyr, V. V., Ponyatovsky, V. A., Djugikowa, E. M., & Shyrobokov V. P., (2015). Modeling of dysbiotic disorders with laboratory animals. Biomedical and Biosocial Anthropology, 24, 230-233.
 Bron, P., Van Baarlen, P., & Kleerebezem M. (2011). Emerging molecular in-sights into the interaction between probiotics and the host intestinal mucosa. Nat. Rev. Microbiol., 10, 66-78.
 Bykov, V. L., (2014). Paneth cells: history of discovery, structural and functional characteristics and the role in the maintenance of homeostasis in the small intestine. Morphology: Archives of anatomy, histology, and embryology, 145(1), 67-80.
 Chang, P. V., Hao, L., Offermanns, S., & Medzhitov, R. (2014). The microbial metabolite butyrate regulates intestinal macrophage function via histone deacetylase inhibition. Proceedings of the National Academy of Sciences of the United States of America, 111(6), 2247-2252. doi: 10.1073/pnas.1322269111
 Dethlefsen, L., & Relman, D. A. (2011). Incomplete recovery and individualized responses of the human distal gut microbiota to repeated antibiotic perturbation. Proc. Natl. Acad. Sci. USA, 108(1), 4554-4561. doi: 10.1073/pnas.1000087107
 Duvallet, C., Gibbons, S. M., Gurry, T., Irizarry, R. A., & Alm, E. J. (2017). Meta-analysis of gut microbiome studies identifies disease-specific and shared responses. Nat. Commun., 8, 1-46. doi: 10.1038/s41467-017-01973-8
 Dykstra, M. J., & Reuss, L. E. (2003). Biological electron microscopy: theory, techniques, and troubleshooting. Springer Science & Business Media.
 Falcony, G., Joossens, M., Vieira-Silva, S., Wang, J., Darzi, Y., Faust, K., … Raes, J. (2016). Population-level analysis of gut microbiome variation. Science, 352(6285), 560-564. doi: 10.1126/science.aad3503
 Johnson, C. D., & Kudsk, K. A. (1999). Nutritional and intestinal mucosal immunity. Clin. Nutr., 18(6), 337-344. doi: https://doi.org/10.1016/S0261-5614(99)80012-0
 Kravchuk, R. I., Sheybak, V. M., Zhmakin, A. I., Goretsky, M. V., & Egorov, A. S. (2007). Characteristics of ultrastructural changes in the mucous membrane of the jejunum after acetaminophen-induced rats dysbacteriosis. Journal of Grodno State Medical University, 1, 106-109.
 Lagier, J. C., Edouard, S., Pagnier, I., Mediannikov, O., Drancourt, M., & Raoult, D. (2015). Current and past strategies for bacterial culture in clinical microbiology. Clinical microbiology reviews, 28(1), 208-236. doi: 10.1128/CMR.00110-14
 Lin, L., & Zhang, J. (2017). Role of intestinal microbiota and metabolites on gut homeostasis and human diseases. BMC immunology, 18(1), 2. doi: 10.1186/s12865-016-0187-3.
 Lloyd-Price, J., Abu-Ali, G., & Huttenhower, C. (2016). The healthy human microbiome. Genome Med., 8(1), 51. doi: 10.1186/s13073-016-0307-y
 Mokili, J. L., Rohwer, F., & Dutilh, B. E. (2012). Metagenomics and future perspectives in virus discovery. Current Opinion in Virology, 2(1), 63-77. doi: 10.1016/j.coviro.2011.12.004
 Moore, H. C., Jacoby, P., Taylor, A., Harnett, G., Bowman, J., Riley, T. V., … Lehmann, D. (2010). The interaction between respiratory viruses and pathogenic bacteria in the upper respiratory tract of asymptomatic Aboriginal and non-Aboriginal children. Pediatr. Infect. Dis. J., 29(6), 540-545. doi: 10.1097/INF.0b013e3181d067cb
 Ovcharova, A. N. (2012). The morphological characteristics of the small and large intestine in experimental primary dysbiosis and its correction by probiotics. (Abstract dis. Cand. Biol. Sciences). Scientific research Institute of Human Morphology, Moscow, RAMS.
 Qin, J., Li, R., Raes, J., Arumugam, M., Burgdorf, K. S., Manichanh, C. … Wang, J. (2010). A human gut microbial gene catalogue established by metagenomic sequencing. Nature, 464, 59-65. doi: 10.1038/nature08821
 Sarkisova, D. S., & Perova, Yu. L. (Edit.) (1996). Microscopic technique. Guide for doctors and laboratory technicians. Moscow: Medicine.
 Shirobokov, V. P., Yankovsky, D. S., & Dyment, G. S. (2014). Microbes in biogeochemical processes, the evolution of the biosphere and the existence of mankind. K.: Veres.
 The Human Microbiome Project Consortium (2012). A framework for human microbiome research. Nature, 486(7402), 215-221. doi: 10.1038/nature11209
 Thursby, E., & Juge, N. (2017). Introduction to the human gut microbiota. The Biochemical journal, 474(11), 1823-1836. doi: 10.1042/BCJ20160510
 Williams, M. D., Ha, C. Y., & Ciorba, M. A. (2010). Probiotics as therapy in gastroenterology: A study of physician opinions and recommendations. J. Clin. Gastroenterol, 44(9), 631-636. doi: 10.1097/MCG.0b013e3181d47f5b
 Yankovsky, D. S., Shirobokov, V. P., Volosovets, А. P., & Моiseenkо, R. А., Dyment, G. S. (2013). Human microbiome and modern methods of its improvement. Journal of the National Academy of Medical Sciences of Ukraine, 19(4), 411-420.
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