Features of the thyroid gland structural components remodeling in the toxemia stage after experimental thermal injury

  • V.G. Koritskiy SHEI “I. Horbachevsky Ternopil State Medical University Ministry of Health of Ukraine”, Ternopil, Ukraine
  • Z.M. Nebesna SHEI “I. Horbachevsky Ternopil State Medical University Ministry of Health of Ukraine”, Ternopil, Ukraine
Keywords: thyroid gland, microscopic and submicroscopic changes, thermal skin injury

Abstract

Thermal injury causes severe structural and metabolic disturbances not only of the skin itself, but also of all organs and systems of the affected organism, is a manifestation of a complex symptom complex - a burn disease. Of particular importance in patients with burns are changes in the endocrine system. The aim of the study was to establish the microscopic and electron-microscopic reorganization of the components of the thyroid gland of animals after thermal damage on 14 day after experimental thermal injury. A III degree burn was applied under ketamine anesthesia with copper plates heated in boiled water to a temperature of 97-100°C. The size of the lesion area was 18-20% of the epilated surface of the body of rats. An experimental study of the structural components of the thyroid gland after a burn injury was performed on laboratory white male rats weighing 160-180 g. Rats euthanasia was performed after ketamine anesthesia by decapitation. In the experiment, the study of the microscopic and submicroscopic state of the follicles and hemocapillaries of the thyroid gland after thermal injury of the III degree. It has been established that in the toxemia stage after the application of the burn injury on 14 day (late toxemia stage), significant destructive and degenerative changes are found in the thyrocytes of the wall of the follicles and hemocapillaries, the organ acquires a macrofollicular structure. The height of thyrocytes decreases, the nuclei and organelles of cytoplasm are significantly damaged, the number and height of microvilli on their apical surface decreases, which negatively affects the cell's secretory cycle and transcapillary organ metabolic processes. The established destructive changes in the blood capillaries and thyrocytes of the follicles are the morphological manifestation of the suppression of the secretory activity of the thyroid gland during thermal injury and corresponds to the hypofunctional state of the organ.

Downloads

Download data is not yet available.

References

[1] Boychuk, T. M., Khodorovskaya, A. A., Chala, K. M., Chernikova, G. M., & Khodorovsky, V. M. (2011). Morphometric indices of functional activity of the thyroid gland under stress reaction. Bukovinsky Medical Bulletin, 15(2), (58), 89-91.
[2] Cherkasov, V. G., Kovalchuk, A. I., Dzevulskaya, I. V., Malikov, A.V., Lakhtadyr, T.V., & Matkivskaya, R. M. (2015). Structural transformations in the internal organs with infusion therapy for burn disease. Medical Science of Ukraine, 11(3-4), 4-11.
[3] Dzevulska, І. V., Kovalchuk, О. І., Cherkasov, E. V., Majewskyi, О. Ye., Shevchuk, Yu. G., Pastukhova, V. A. & Kyselova, T. M. (2018). Influence of lactoproteinum solution with sorbitol on dna content of cells of endocrine glands on the background of skin burn in rats. World of Medicine and Biology, 64(2), 033-039. doi: 10.26724/2079-8334-2018-2-64-33-39
[4] Dzevulska, I. V. (2015). Monthly rates of cell cycle of rat adrenal cells in administration of 0,9% NaCl solution, Lactoprotein with sorbitol or HAES-LX-5% during the first 7 day. Biomedical and Biosocial Anthropology, 25, 33-37.
[5] Evers, L. H. (2010). The biology of burn injury. Exp. Dermatol, 19(2), 9, 777-783 doi:10.1111/j.1600-0625.2010.01105.x
[6] Farina, Jr., J. A., Rosique, M. J., & Rosique, R. G. (2013). Curbing inflammation in burn patients. International Journal of Inflammation, 2013, 1-9. doi: 10.1155/2013/715645
[7] Galunko, G. М. (2017). Histological changes in the small intestine in the advanced stages of burn disease. World of Medicine and Biology, 3(61), 90-96. doi: 10.26724 / 2079-8334-2017-3-61-90-96
[8] Gunas, I., Dovgan, I., & Masur, O. (1997). Method of thermal burn trauma correction by means of cryoinfluence. Abstracts are presented in zusammen mit der Polish Anatomical Society with the participation of the Association des Anatomistes Verhandlungen der Anatomischen Gesellschaft, Olsztyn (р. 105). Jena – München: Der Urban & Fischer Verlag.
[9] Goralskiy, L. P., Homich, V. Т., & Kononskiy, О. І. (2011). Fundamentals of histological technique and morphofunctional methods of research in norm and in pathology. Zhitomir: Polissya.
[10] Gunas, I. V., Guminskiy, Yu. I., Ocheretna, N. P., Lysenko, D. A., Kovalchuk, О. І., Dzevulska, І. V., & Cherkasov, E. V. (2018). Indicators cell cycle and dna fragmentation of spleen cells in early terms after thermal burns of skin at the background of introduction 0.9% NaCl solution. World of Medicine and Biology, 1(63), 116-120. doi: 10.26.724/2079-8334-2018-1-63-116-120
[11] Gunas, I. V., Kovalchuk, O. I., Cherkasov, V. G., & Dzevulskaya, I. V. (2014). Structural aspects of the organs adaptive changes of the neuroimundocrine system in the treatment of burn disease with combined hyperosmolar solutions. Galician Medical Herald, 21(2), 21-26.
[12] Janak, J. C., Clemens, M. S., Howard, J. T., Le T. D., Cancio, L. C., Chung, K. K., & Stewart, Ian J. (2018). Using the injury severity score to adjust for comorbid trauma may be double counting burns: implications for burn research. Burns, 44, 8, 1920-1929. doi: 10.1016/j.burns.2018.03.012
[13] Jeschke, M. G., Pinto, R., & Kraft, R. (2015). Inflammation and the Host Response to Injury Collaborative Research Program. Morbidity and survival probability in burn patients in modern burn care. Crit. Care. Med, 43(2), 4, 808-815.
[14] Kallinen, O., Maisniemi, K., Bohling, T., Tukiainen, E., & Koljonen, V. (2012). Multiple organ failure as a cause of death in patients with severe burns. J. Burn Care Res., 33(2), 206-211. doi: 10.1097/BCR.0b013e3182331e73
[15] Kearney, L., Francis, E. C., & Clover, A. J. (2018). New technologies in global burn care - a review of recent advances. Int. J. Burns Trauma, 8(4), 77-87. PMID: 30245912
[16] Kovalchuk, O. I. (2016). Features of cell cycle indices in the adenohypophysis at late terms post-burn skin injury in rats under separate infusion in the first 7 days of 0.9% solution of NaCl, lactobacillus solutions with sorbitol or HAES-LX-5%. Ukrainian Scientific Medical Youth Journal, 93(1), 24-31.
[17] Maden, М. (2018). Optimal skin regeneration after full thickness thermal burn injury in the spiny mouse. Acomys cahirinus. Burns, 44, 6, 1509-1520. doi: 10.1016/j.burns.2018.05.018
[18] Nurmetova, I. K., & Kuhar, I. D. (2012). Organometric parameters of thyroid gland in rats with acute burned toxemia on the background of treatment with infusion drugs. Ukrainian Journal of Hematology and Transfusiology, 15(4), 278-281.
[19] Nurmetova, I. K. (2012). Morphometric parameters of the thyroid gland during thermal trauma in the dynamics of its experimental treatment with combined hyperosmolar solutions on the 21st and 30th day of the experiment. Reports of Morphology, 18(2), 263-265.
[20] Nebesna, Z. M., Lisnichuk, N. Ye., & Demkiv, I. Ya. (2015). Dynamics of changes of oxidation-reduction reactions in the lung tissue in case of burn injury and its correction by lyophilized xenograft substrate. Bulletin of Biology and Medicine, 4(1), (124), 124-128.
[21] Netyukhailo, L. G., Kharchenko, A. G., & Kostenko, S. V. (2011). Pathogenesis of burn disease (in 2 parts). World of Medicine and Biology, 1, 127-131, 131-135.
[22] Regas, F. C., & Ehrlich, H. P. (1992). Elucidating the vascular response to burns with a new rat model. J. Trauma, 32, 5, 557-563.
[23] Strelchenko, Yu. I., Zyablytsev, S. V., & Yale, V. M. (2012). Pathophysiological relationships of pituitary-thyroid and pituitary-adrenal systems under the influence of polarized light in rats with dosed burns to open flames. Clinical and Experimental Pathology, 11(2), 3, 156-158.
[24] Swanson, J. W., Otto, A. M., Gibran, N. S., Klein, M. B., Kramer, C. B., Heimbach, D. M., & Pham, T. N. (2013). Trajectories to death in patients with burn injury. J. Trauma Acute Care Surg., 74(1), 282-288. doi: 10.1097/TA.0b013e3182788a1c
[25] Takayuki, Y., Hayato, I., Timothy, W., King, H., Hara, D., & Cooper, K. (2018). Skin xenotransplantation: Historical review and clinical potential. Burns, 44(8), 1738-1749, doi: 10.1016/j.burns.2018.02.029
[26] Timmers, T. K., Verhofstad, M. H., & Leenen, L. P. (2015). Intensive care organisation: Should there be a separate intensive care unit for critically injured patients? World Journal of Critical Care Medicine, 4, 240-243. doi:10.5492/wjccm.v4.i3.240
Published
2018-09-27
How to Cite
Koritskiy, V., & Nebesna, Z. (2018). Features of the thyroid gland structural components remodeling in the toxemia stage after experimental thermal injury. Reports of Morphology, 24(3), 37-42. https://doi.org/10.31393/morphology-journal-2018-24(3)-06