Morphometric studies of the damaged skin area after experimental thermal trauma and during correction with a cryo-lyophilized xenograft skin substrate

  • S.B. Kramar Ivan Horbachevsky Ternopil State Medical University, Ternopil, Ukraine
  • K.S. Volkov Ivan Horbachevsky Ternopil State Medical University, Ternopil, Ukraine
  • Z.M. Nebesna Ivan Horbachevsky Ternopil State Medical University, Ternopil, Ukraine
Keywords: skin, thermal trauma, cryoliophilized xenodermal substrate, morphometric studies


According to WHO, burns rank third place among other injuries, and in some countries, the second, after traffic injuries. One of the promising tools for treating burn wounds is the use of lyophilized xenograft skin substrate. The purpose of this work was to determine the morphometric parameters of the affected by burns area of the skin in the dynamics after an experimental thermal trauma and in the case of correction by crushed lyophilized xenograft skin substrate. Burning of third degree on the shaved skin of the back of the guinea pig was applied to the vapor under general anesthetic. Morphometrically, at 7, 14, and 21 days of the experiment, the thickness of the epidermis (thin skin without a layer of scales), the number of fibroblast cells per unit area, the outer and inner diameter of the capillaries were determined in the boundary and central areas of the wound. Morphometric studies were carried out using programs VideoTest-5.0, KAAPA Image Base and Microsoft Exсel on a personal computer. Statistical processing of the obtained quantitative data was carried out using methods of variation statistics with the determination of the mean arithmetic and its error (M±m), Student’s criterion (t) and reliability index (p). Differences are considered valid at р≤0.05. It has been established that already in the 7 day of the experiment, under conditions of wound closure by xenograft skin substrate marked a significant thickening of the epidermis on the periphery of the wound appear. Activation of the process of boundary epithelization contributes to the renewal of components of the microcirculatory bed, the formation of granulation tissue. It was found that on the 14 day of application of the corrective factor in the peripheral zone of the wound, the number of cells of the fibroblast row and the mean value of the thickness of the epidermis reach their peak value, significantly (p<0.001) exceeding such indices of animals in intact group and group of animals without correction. The morphometric parameters of capillaries in this period of the experiment indicate a good development of the microcirculation, which improves regional epithelization. On the 21 day of the experiment, under the condition of correction, the border between the regional and central parts of the wound is almost lost. Thus, the results of morphometric studies indicate that the use of cryo-lyophilized xenograft skin substrate after a thermal trauma of the skin contributes to the healing of the wound defect with the formation of connective tissue of the dermis, angiogenesis, and complete epithelization of the surface of the affected epidermis layer.


[1] Adeloye, D., Bowman, K., Chan, K. Y., Patel, S., Campbell, H., & Rudan, I. (2018). Global and regional child deaths due to injuries: an assessment of the evidence. J. Glob. Health, 8(2), 021104. doi: 10.7189/jogh.08.021104.
[2] Andrade, P., Kaura, A. S., Bryant, J. R., & Burke, E. (2018). Thermal Burn Injury from a Wedding Ring: An Unusual Case. J. Am. Coll. Clin. Wound Spec., 9(1-3), 32-34. doi: 10.1016/j.jccw.2018.06.004
[3] Avtandilov, H. H. (2002). Basics of quantitative pathological anatomy. Moskow: Medicine.
[4] Bihuniak, V. V., & Povstianyi, M. Yu. (2004). Termal injury. Ternopil: Ukrmedknuha.
[5] Han, C. M., Yu, M. R., & Wang, X. G. (2018). Summary of advances in the research of wound therapy. Zhonghua Shao Shang Za Zhi., 34(12), 864-867. doi: 10.3760/cma.j.issn.1009-2587.2018.12.009.
[6] Hanglin, Ye., & Suvranu, D. (2017). Thermal injury of skin and subcutaneous tissues: A review of experimental approaches and numerical models. Burns, 43(5), 909-932. doi:10.1016/j.burns.2016.11.014.
[7] Horalskyi, L. P., Khomych, V. T., & Kononskyi, O. I. (2005). Fundamentals of histological technology and morphofunctional methods of research in norm and in pathology. Zhytomyr: Polissia.
[8] Huda, N. V., & Bihuniak A. V. (2007). The usege of lyophilized xenoderm transplantants for the treatment of derma burns in the elderly. Hospital surgery, 3, 81-83.
[9] Huda, N. V., & Tsymbaliuk, A. V. (2012). Content of aminoacids and microelements in crioliofilized xenoskin as an indicator of its biological activity. Medical chemistry, 14(1), 50, 70-72.
[10] John, P. A., John, S., John, G., Sparrow, E., & Minkowycz, W. J. (2018). Tissue burns due to contact between a skin surface and highly conducting metallic media in the presence of inter-tissue boiling. Burns.
[11] Kovalchuk, A. O., & Piatkovskyi, T. I. (2010). Dynamical changes of results of experimental burn wounds after early necrectomy using lyophilized xenodermotransplants of secondary cutting. Reports of scientific researches, 2, 46-49.
[12] Lee, J. H., Kim, J. W., Lee, J. H., Chung, K. J., Kim, T. G., Kim, Y. H., & Kim, K. J. (2018). Wound healing effects of paste type acellular dermal matrix subcutaneous injection. Arch Plast Surg., 45(6), 504-511. doi: 10.5999/aps.2018.00948.
[13] Leszczynska, A., Kulkarni, M., Ljubimov, A. V., & Saghizadeh, M. (2018). Exosomes from normal and diabetic human corneolimbal keratocytes differentially regulate migration, proliferation and marker expression of limbal epithelial cells. Sci. Rep., 8(1), 15173. doi: 10.1038/s41598-018-33169-5.
[14] Liu, Y. C., Ang, H. P., Teo, E. P., Lwin, N. C., Yam, G. H., & Mehta, J. S. (2016). Wound healing profiles of hyperopic-small incision lenticule extraction (SMILE). Sci. Rep., 6, 29802. doi: 10.1038/srep29802.
[15] Miadelets, O. D., & Adaskevich, V. P. (2006). Morphofunctional dermatology. Moskow: Medlit.
[16] Sarkisov, D. S., & Perova, Yu. L. (1996). Microscopic technique. Moskow: Medcine.
[17] Sarrazy, V., Billet, F., Micallef, L., Coulomb, B., & Desmouliere, A. (2011). Mechanisms of pathological scarring: Role of myofibroblasts and current developments. Wound Repair and Regeneration, 19 (1), 10-15. doi: 10.1111/j.1524-475X.2011.00708.x.
[18] Smeringaiova, I., Reinstein Merjava, S., Stranak, Z., Studeny, P., Bednar, J., & Jirsova, K. (2018). Endothelial Wound Repair of the Organ-Cultured Porcine Corneas. Curr. Eye Res., 43(7), 856-865. doi: 10.1080/02713683.2018.1458883.
[19] Viter, V. S. (2014). Ultrastructural state of muscular tunic of the heart after experimental thermal injury in applying lyophilized xenografts. Nauka i Studia, 8 (118), 107-111.
[20] Willenborg, S., & Eming, S. A. (2018). Cellular networks in wound healing. Science, 362 (6417), 891-892. doi: 10.1126/science.aav5542.
[21] Woodley, D. T. (2017). Distinct Fibroblasts in the Papillary and Reticular Dermis: Implications for Wound Healing. Dermatol. Clin., 35(1), 95-100. doi: 10.1016/j.det.2016.07.004.
[22] Xiao, W., Tang, H., Wu, M., Liao, Y., Li, K., Li, L., & Xu, X. (2017). Ozone oil promotes wound healing by increasing the migration of fibroblasts via PI3K/Akt/mTOR signaling pathway. Biosci. Rep., 37(6). pii: BSR20170658. doi: 10.1042/BSR20170658.
[23] Yoon, D., Yoon, D., Sim, H., Hwang, I., Lee, J. S., & Chun, W. (2018). Accelerated Wound Healing by Fibroblasts Differentiated from Human Embryonic Stem Cell-Derived Mesenchymal Stem Cells in a Pressure Ulcer Animal Model. Stem Cells Int., 30, 4789568 doi: 10.1155/2018/4789568
[24] Zviahintseva, T. V., Hrun, V. V., & Naumova, O. V. (2013). Morphological changes in the skin of guinea pigs after local ultraviolet irradiation with the use of drugs with photoprotective activity. Medicine today and tomorrow, 58(1), 59-63.
How to Cite
Kramar, S., Volkov, K., & Nebesna, Z. (2018). Morphometric studies of the damaged skin area after experimental thermal trauma and during correction with a cryo-lyophilized xenograft skin substrate. Reports of Morphology, 24(4), 22-28.