Structural changes of duodenal mucosa enterocytes of rats in burn skin injury under experimental streptozotocin-induced diabetes mellitus
The work is devoted to the study of structural changes of enterocytes in the mucous membrane of the duodenum in a burn injury of the skin of a rat under conditions of experimental streptozotocin induced diabetes. The study was carried out on laboratory white adult rats-males weighing 180-210 g. The control group consisted of 21 animals without somatic pathology, the first experimental group consisted of 21 rats with skin burn injury, the second experimental group cleared 21 rats with skin burn and experimental streptozotocin induced diabetes. A model of experimental diabetes mellitus was reproduced by administering streptozotocin intraperitoneally once in dose of 50 mg/kg to rats. In an experimental simulation of a skin burn, two copper plates in the form of an ellipse were kept in water at 100oC for 10 minutes and, under ether anesthesia conditions, were applied simultaneously symmetrically on both exposed parts of the body of rats with an exposure for 10 seconds. Burn skin damage in rats was II-AB degrees – dermal surface burn (according to the old classification III-A degree) with a total area of 21-23% of the body surface with the development of burn shock. For morphological studies, the duodenum was taken, fragments of which were processed using conventional light and electron microscopy. The main criteria for assessing damage the enterocytes of the duodenal mucosa were the results studies of histological and ultrastructural data over 7, 14 and 21 days after a skin burn. The results of the studies showed that the damage of the enterocytes of the duodenal mucosa is based on deep destructive changes, which after 21 days (at the stage of septic toxemia), as a rule, are not reversible and develop against the background of significant intoxication of the organism. In the mucous membrane of the duodenum with burn injury of the skin associated with diabetes mellitus, there is a deterioration of the manifestations of the adaptive response and prolongation of destructive processes, accompanied by a violation of intercellular interactions in cytoarchitectically modified and deformed villi and crypts.
 Cherkasov, V. G., Kovalchuk, A. I., Dzevulskaya, I. V., Malikov, A. V., Lakhtadyr, T. V., & Matkivskaya, R. M. (2015). Structural transformations in the internal organs with infusion therapy for burn disease. Medical Science of Ukraine, 11(3-4), 4-11.
 Dzevulska, І. V., Kovalchuk, О. І., Cherkasov, E. V., Majewskyі, О. Ye., Shevchuk, Yu. G., Pastukhova, V. A., & Kyselova, T. M. (2018). Influence of lactoproteinum solution with sorbitol on DNA content of cells of endocrine glands on the background of skin burn in rats. World of Medicine and Biology, 64 (2), 33-39. doi: 10.26724/2079-8334-2018-2-64-33-39
 Evers, L. H. (2010). The biology of burn injury. Exp. Dermatol., 19(2), 9, 777-783. doi: 10. 1111/j.1600-0625.2010.01105.x
 Galunko, G. M. (2017). Histological changes in the small intestine in the advanced stages of burn diseases. World of Medicine and Biology, 3(61), 90-96. doi: 10.26724/ 2079-8334-2017-3-61-90-96
 Gotfried, J., Priest, S., & Schey, R. (2017). Diabetes and the small intestine. Curr. Treat. Options. Gastroenterol., 15(4), 490-507. doi: 10.1007/s11938-017-0155-x
 Gunas, I., Dovgan, I, & Masur, O. (1997). Method of intermal burn trauma correction by means of cryoinfluence. Abstracts are presented in zusammen mit der Polish Anatomical Society with the participation of the Association des Anatomistes Verhandlungen der Anatomishen Geselleschaft, Olsztyn (p. 105). Jena-Munchen: Der Urban & Fisher Verlag.
 Gunas, I. V., Guminskiy, Yu. I., Ocheretna, N. P., Lysenko, D. A., Kovalchuk, O. I., Dzevulska, I. V., & Cherkasov, E. V. (2018). Indicators cell cycle and DNA fragmentation of spleen cells in early terms after thermal burns of skin at the background of introduction 0.9% NaCl solution. World of Medicine and Biology, 1(63), 116-120. doi: 10.26.724/2079-8334-2018-1-63-116-120
 Gunas, I. V., Kovalchuk, O. I., Cherkasov, V. G., & Dzevulska, I. V. (2014). Structural aspects of the organs adaptive changes of the neuroimundocrine system in the treatment of burn diseases with combined hyperosmolar solutions. Galician Medical Herald, 21(2), 21-26.
 Haagsma, J. A., Greatz, N., & Bolliger, I. (2016). The global burden of injury: incidence, mortality, disability – adjusted life years and time trends from the Global Burden of Diseases study 2013. Injury Prevention, 22(1), 3-18.
 Huang, Ya., Feng, Ya., Wang, Yu., Wang, Pei., Wang, Fe., & Ren, Hui. (2018). Severe burn-induced intestinal epithelial barrier dysfunction is associated with endoplasmic reticulum stress and autophagy in mice. Front Physiol., 9, 441. doi: 10.3389/fphys.2018.00441
 Jeschke, M. G., Pinto, R., & Kraft, R. (2015). Inflammation and the Host Response to Injury Collaborative Research Program. Morbidity and survival probability in burn patients in modern burn care. Crit. Care. Med., 43(2), 4, 808-815. NCT00257244
 Kallinen, O., Maisniemi, K., Bohling, T., Tukiainen, E., & Koljonen, V. (2012). Multiple organ failure as a cause of death in patients with severe burns. J. Burn Care Res., 33(2), 206-211. doi: 10.1097/BCR.0b013e3182331e73
 Kearney, L., Francis, E. C., & Clover, A. J. (2018). New technologies in global burn care – a review of recent advances. Int. J. Burns Trauma, 8(4), 77-87. PMID: 30245912
 Natrus, L.V., Ryzhko, I. N., Kozak, A. I., Kryvosheieva, O. I., & Stechenko, L. A. (2017). Ultrastructural base of the connective tissue skin' cells interactions at burn injury in the hyperglycemic white rats. World of Medicine and Biology, 4(62), 157-162. doi: 10.26724/2079-83342017-4-62-157-162
 Netyukhailo, L. G., Kharchenko, A. G., & Kostenko, S. V. (2011). Pathogenesis of burn disease (in 2 parts). World of Medicine and Biology, 1, 127-131, 131-135.
 Regas, F. C., & Ehrlich, H. P. (1992). Elucidating the vascular response to burns with a new rat model. J. Trauma, 32(5), 557-563.
 Smolle, Ch., Cambiaso-Daniel, J., & Forbes, A. A. (2017). Recent trends in burn epidemiology woredwide: A systemic review. Burns, 43(2), 249-257. doi: 10.1016/j.burns.2016.08.013.
 Swanson, J. W., Otto, A. M., Gibran, N. S., Klein, M. B., Kramer, C. B., Heimbach, D. M., & Pham, T. N. (2013). Trajectories to death in patients with burn injury. J. Trauma Acute Care Surg., 74(1), 282-288. doi: 10.1097/TA.0b013e3182788a1c
 Timmers, T. K., Verhofstad, M. H., & Leenen, L. P. (2015). Intensive care organisation: Should there be a separate intensive care unit for critically injured patients? World Journal of Critical Care Medicine, 4, 240-243. doi: 10.5492/wjccm.v4.i3.240
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