Submicroscopic changes of alveoli of respiratory department of lungs a day after experimental thermal trauma

  • Z.M. Nebesna Ivan Horbachevsky Ternopil National Medical University of the Ministry of Health of Ukraine, Ternopil, Ukraine
  • O.I. Bashynska National Pirogov Memorial Medical University, Vinnytsya, Ukraine
  • N.P. Ocheretna National Pirogov Memorial Medical University, Vinnytsya, Ukraine
  • G.М. Galunko National Pirogov Memorial Medical University, Vinnytsya, Ukraine
  • O.Ya. Slyvka National Pirogov Memorial Medical University, Vinnytsya, Ukraine
Keywords: lung alveoli, aerohematic barrier, submicroscopic changes, thermal trauma.


Deep, large thermal burns are not limited to local lesions of tissues, they cause significant disruption of all systems and organs of the organism, change in metabolic processes. It is revealed that the primary links in the pathogenesis of burn disease are destruction of the skin, impaired neuroendocrine regulation and significant hemodynamic disorders. The reorganization of structures and impaired lung function, in response to a pathological process in the body, is attracting increasing attention of scientists. The aim of the study was to establish a submicroscopic rearrangement of the alveoli after a thermal lesion for 1 day after the experimental thermal trauma. Grade III burns were applied under ketamine anesthesia with copper plates heated in boiled water to a temperature of 97-1000С. The size of the lesion area was 18-20 % of the epilated surface of the body of rats. An experimental study of the structural components of lung alveoli after burn injury was performed on laboratory white male rats weighing 160-180 g. Euthanasia of rats was performed after ketamine anesthesia by decapitation. In the experiment, the study of the submicroscopic state of the walls of the alveoli of the lungs after thermal trauma was done. It is established that in the stage of shock after the application of burn injury – 1 day, in the alveoli of the respiratory department of the lung, there are adaptive compensatory and initial destructive changes of all structural components of the alveoli. Damage to the ultrastructure of the aerohematical barrier is manifested by intracellular edema and edema of the organelles of the endothelial cells, respiratory and secretory epitheliocytes, and the amount of heterochromatin increases in their deformed nuclei. The basement membrane also has signs of edema, sometimes homogeneous, fuzzy. The decrease in the number of vesicles and micropinocytotic vesicles in endothelial and respiratory epitheliocytes leads to impaired endothelial and alveolar metabolism. Numerous actively phagocytic alveolar macrophages with a well-expressed lysosomal apparatus are found in the alveoli. Initial alternative alterations of the ultrastructure of the components of the air-barrier barrier lead to disruption of gas exchange in the respiratory department of the lungs


[1] Cherkasov, V. G., Kovalchuk, A. I., Dzevulskaya, I. V., Malikov, A. V., Lakhtadyr, T. V., & Matkivskaya, R. M. (2015). Structural transformations in the internal organs with infusion therapy for burn disease. Medical science of Ukraine, 11(3-4), 4-11.

[2] Dzevulska, І. V., Kovalchuk, О. І., Cherkasov, E. V., Majewskyi, О. Ye., Shevchuk, Yu. G., Pastukhova, V. A., & Kyselova, T. M. (2018). Influence of lactoproteinum solution with sorbitol on dna content of cells of endocrine glands on the background of skin burn in rats. World of Medicine and Biology, 2(64), 33-39. doi:10.26724/2079-8334-2018-2-64-33-39

[3] Evers, L. H., Bhavsar, D., & Mailänder, P. (2010). The biology of burn injury. Experimental dermatology, 19(9), 777-783. doi: 10.1111/j.1600-0625.2010.01105.x

[4] Gavryluk, А. О., Gunas, I. V., Galunko, G. М., Chereshniuk, І. L., & Lysenko, D. A. (2017). Indicators of the cell cycle and fragmentation of DNA of cells of small intestinal mucosa through 14, 21 and 30 days after burn skin damage on the background of infusion of 0,9 % NaCl solution. Biomedical and Biosocial Аnthropology, 29, 104-108.

[5] Goralskiy, L. P., Homich, V. Т., & Kononskiy, О. І. (2011). Fundamentals of histological technique and morphofunctional methods of research in normal and in pathology. Zhitomir: Polissya.

[6] Gunas, I. V., Guminskiy, Yu. I., Ocheretna, N. P., Lysenko, D. A., Kovalchuk, O. І., Dzevulska, I. V., & Cherkasov, E. V. (2018). Indicators cell cycle and dna fragmentation of spleen cells in early terms after thermal burns of skin at the background of introduction 0.9 % NaCl solution. World of Medicine and Biology, 1(63), 116-120. doi: 10.26.724/2079-8334-2018-1-63-116-120

[7] Herold, S., Gabrielli, N. M., & Vadász, I. (2013). Novel concepts of acute lung injury and alveolar-capillary barrier dysfunction. American Journal of Physiology-Lung Cellular and Molecular Physiology, 305(10), L665-L681. doi: 10.1152/ajplung.00232.2013

[8] Janak, J. C., Clemens, M. S., Howard, J. T., Le, T. D., Cancio, L. C., Chung, K. K., ... Stewart, I. J. (2018). Using the injury severity score to adjust for comorbid trauma may be double counting burns: implications for burn research. Burns, 44(8), 1920-1929. doi: 10.1016/j.burns.2018.03.012

[9] Jeschke, M. G., Pinto, R., Kraft, R., Nathens, A. B., Finnerty, C. C., Gamelli, R. L., ... Herndon, D. N. (2015). Morbidity and survival probability in burn patients in modern burn care. Critical care medicine, 43(4), 808-815. doi: 10.1097/CCM.0000000000000790

[10] Kallinen, O., Maisniemi, K., Böhling, T., Tukiainen, E., & Koljonen, V. (2012). Multiple organ failure as a cause of death in patients with severe burns. Journal of burn care & research, 33(2), 206-211. doi: 10.1097/BCR.0b013e3182331e73

[11] Kearney, L., Francis, E. C., & Clover, A. J. (2018). New technologies in global burn care-a review of recent advances. International journal of burns and trauma, 8(4), 77-87. PMID: 30245912

[12] Maievskyi, O. Y., Bobruk, S. V., & Gunas, I. V. (2018). Dynamics of histochemical changes in the skin of rats within a month after the burning of II-III degrees on the background of the injection first 7 days HAES-LX-5% solution. World of Medicine and Biology, 4(66), 180-184. doi: 10.26724/2079-8334-2018-4-66-180-184

[13] Netyukhailo, L. G., Kharchenko, A. G., & Kostenko, S. V. (2011). Pathogenesis of burn disease (in 2 parts). World of Medicine and Biology, 1, 127-131.

[14] Ocheretnyuk, A. O., Palamarchuk, O. V., Lysenko, D. A., Vashchuk, G. I., & Stepanyuk, G. I. (2018). Influence of solution of lactoprotein with sorbitol on ultrastructural changes in lungs of rats in the condition of burn shock. Regulatory Mechanisms in Biosystems, 9(3), 440-445.

[15] Porter, C., Tompkins, R. G., Finnerty, C. C., Sidossis, L. S., Suman, O. E., & Herndon, D. N. (2016). The metabolic stress response to burn trauma: current understanding and therapies. The Lancet, 388(10052), 1417-1426. doi: 10.1016/S0140-6736(16)31469-6

[16] Rowan, M. P., Cancio, L. C., Elster, E. A., Burmeister, D. M., Rose, L. F., Natesan, S., ... & Chung, K. K. (2015). Burn wound healing and treatment: review and advancements. Critical care, 19(1), 243. doi: 10.1186/s13054-015-0961-2

[17] Sousse, L. E., Herndon, D. N., Andersen, C. R., Zovath, A., Finnerty, C. C., Mlcak, R. P., ... & Hawkins, H. K. (2015). Pulmonary histopathologic abnormalities and predictor variables in autopsies of burned pediatric patients. Burns, 41(3), 519-527. doi: 10.1016/j.burns.2014.09.014

[18] Tiwari, V. K. (2012). Burn wound: How it differs from other wounds? Indian journal of plastic surgery, 45(02), 364-373. doi: 10.4103/0970-0358.101319

[19] Zhang, D., Chang, Y., Han, S., Yang, L., Hu, Q., Yu, Y., ... & Chai, J. (2018). The microRNA expression profile in rat lung tissue early after burn injury. Turkish Journal of Trauma and Emergency Surgery, 24(3), 191-198. doi: 10.5505/tjtes.2018.98123
How to Cite
Nebesna, Z., Bashynska, O., Ocheretna, N., Galunko, G., & Slyvka, O. (2019). Submicroscopic changes of alveoli of respiratory department of lungs a day after experimental thermal trauma. Reports of Morphology, 25(3), 16-20.