Histological picture in the skin of rats within a month after burn II-III degree against the background of the introduction for first 7 days 0.9% NaCl solution

  • Ye.V. Mironov National Pirogov Memorial Medical University, Vinnytsya, Ukraine
Keywords: skin burns, histological changes, necrosis, 0.9% NaCL solution.


Burn disease is a complex of pathological changes that occur in the body due to the action of a thermal agent and are life-threatening. The problem of skin burns still remains relevant today. Insufficiently studied features of pathogenesis and methods of treatment of thermal trauma are the cause of considerable interest of scientists in this problem. The aim is to study the features of microscopic changes in the skin of rats during the month after grade II-III burn on the background of the introduction of the first 7 days of 0.9% NaCL solution. The studies were performed on 360 laboratory white male rats weighing 155-160 g. During the experiment, the animals were divided into 4 groups: 1st, 2nd groups – rats without thermal trauma infused with 0.9% NaCl solution and HAES-LX-5% at a dose of 10 ml/kg. In the 3rd, 4th groups, rats were infused with 0.9% NaCl solution and HAES-LX-5% at a dose of 10 ml/kg after skin burns. Burning skin damage was caused by applying to the lateral surfaces of the trunk of rats for 10 seconds four copper plates, heated in water at a constant temperature of 100°C. Histological preparations were prepared by standard procedure and examined using an OLYMPUS BH-2 light microscope. Conducted microscopic studies of the skin of animals after thermal trauma under the conditions of application of 0.9% NaCl solution found that in the early stages of the experiment (1, 3, 7 days) compensatory and adaptive changes of its structural components are combined with signs of destructive disorders. In the later periods of thermal trauma (14, 21 and 30 days), the destructive changes of the structural components of the skin in the lesion area deepen, and the process becomes irreversible.


[1] Abdullahi, A., Amini-Nik, S., & Jeschke, M. G. (2014). Animal models in burn research. Cell Mol. Life Sci., 71(17), 3241-3255. doi: 10.1007/s00018-014-1612-5

[2] Bebeshko, N. P. (2013). Dynamics of morphological changes in the spleen of rats at 1, 3 and 7 days after skin burn with the introduction of saline NaCL or lactoprotein with sorbitol. Ukrainian Medical Almanac, 16(3), 12-18.

[3] Butcher, M., & Swales, B. (2012). Assessment and management of patients with burns. Nurs Stand., 27(2), 50-55. doi: 10.7748/ns2012.

[4] Cherkasov, V. G., Gunas, I. V., Kovalchuk, A. I., Dzevulska, I. V., Cherkasov, E. V., Malykov, A. V. … Matkyvskaia, R. M. (2015). Ultrastructural transformations of the intercellular substance in the internal organs in the treatment of burn disease by infusion of combined hyperosmolar solutions. Clinical Аnatomy and Surgical Surgery, 14(1), 37-44.

[5] Dzevulska, I. V. (2015). Monthly dynamics of the ultrastructural changes of the adrenal glands of rats after thermal injury of the skin under the conditions of the first seven days of 0.9% NaCl solution. World of medicine and biology, 3(52), 91-98.

[6] Guilabert, P., Usua, G., Martin, N., Abarca, L., Barret, J. P., & Colomina, M. J. (2016). Fluid resuscitation management in patients with burns: update. Br. J. Anaesth., 117(3), 284-296. doi: 10.1093/bja/aew266

[7] Gunas, I., Dovgan, I., & Masur, O. (1997). Method of thermal burn trauma correction by means of cryoinfluence. Abstracts are presented in zusammen mit der Polish Anatomical Society with the participation of the Association des Anatomistes Verhandlungen der Anatomischen Gesellschaft, Olsztyn (р. 105). Jena – München: Der Urban & Fischer Verlag.

[8] Horalskyi, L. P., Khomych, V. T., & Kononskyi, O. I. (2011). Fundamentals of histological technique and morphofunctional methods of research in normal and pathology. Zhytomyr: Polissya.

[9] Hur, J., Yang, H. T., Chun, W., Kim, J., Shin, S., Kang, H. L., & Kim, H. S. (2015). Inflammatory cytokines and their prognostic ability in cases of major burn injury. Ann. Lab. Med., 35(1), 105-110. doi: 10.3343/alm.2015.35.1.105

[10] Kallinen, O., Maisniemi, K., & Boheing, K. (2012). Multiple organ failure as cause of death in patients with severe burns. J. Burn Care Res., 33(2), 206-211. doi: 10.1097/BCR.0b013e3182331e73

[11] Klychnykova, E. V., Tazyna, E. V., Smyrnov, S. V., Spyrydonova, T. G., Zhyrkova, E. A., Borysov, V. S., & Godkov, M. A. (2015). Interrelation of biochemical indicators of oxidative stress, endogenous intoxication and regulation of vascular tone in patients with burn injury. Anesthesiology and Intensive Care, (1), 45-49.

[12] Kovalenko, O. M. (2014). Issues of infusion therapy for burn shock. Surgery of Ukraine, (2), 13-19.

[13] Kryzyna, P. S., & Pysmenna, O. V. (2011). Pathomorphological changes in the burn wound. Ukraine, Nation’s Health, 1(17), 93-97.

[14] Makarova, O. I. (2013). Histological picture of changes in the lungs of rats at 14, 21 and 30 days after thermal burn of the skin. Biomedical and Biosocial Anthropology, 21, 73-79.

[15] Masood, R. A., Wain, Z. N., Tariq, R., Ullah, M. A., & Bashir, I. (2016). Burn Cases, Their Management and Complications: A Review. International Current Pharmaceutical Journal, 5(12), 103-105. doi: 10.3329/icpj.v5i12.30411

[16] Porter, C., Herndon, D. N., Bhattarai, N., Ogunbileje, J. O., Szczesny, B., Szabo, C. … Sidossis, L. S. (2015). Severe Burn Injury Induces Thermogenically Functional Mitochondria in Murine White Adipose Tissue. Shock, 44(3), 258-264. doi: 10.1097/SHK.0000000000000410

[17] Rowan, M. P., Cancio, L. R., Elster, E. A., Burmeister, D. M., Rose, L. F., Natesan, S. … & Chung, K. K. (2015). Burn wound healing and treatment: review and advancements. Crit. Care, (19), 1-12. doi: 10.1186/s13054-015-0961-2

[18] Saraf, M. K., Herndon, D., N., Porter, C., Toliver-Kinsky, T., Radhakrishnan, R., Chao, T. … Sidossis, L. S. (2016). Morphological Changes in Subcutaneous White Adipose Tissue After Severe Burn Injury. J. Burn Care Res., 37(2), 96-103. doi: 10.1097/BCR.0000000000000292

[19] Szczesny, B., Brunyanszki, A., Ahmad, A., Olah, G., Porter, C., Toliver-Kinsky, T. … Szabo, C. (2015). Time-Dependent and Organ Specific Changes in Mitochondrial Function, Mitochondrial DNA Integrity, Oxidative Stress and Mononuclear Cell Infiltration in a Mouse Model of Burn Injury. PLoS ONE, 10(12), e0143730. doi: 10.1371/journal.pone.0143730

[20] Vons, В. V., Chubka, М. B., & Groshovyi, T. A. (2018). The problem of burn injury treatment and drug characteristics for local burn treatment. Topical issues in pharmaceutical and medical science and practice, 11, 1(26), 119-125. https://doi.org/10.14739/2409-2932.2018.1.123731

[21] Yvchenko, E. V., Borysov, D. N., Golota, A. S., Krassyi, A. B., & Rusev, I. T. (2015). Combined burns in the structure of modern civil and combat burn injury. Military Medical Journal, 336(2), 22-25.

[22] Zviahyntseva, T. V., Kryvoshapka, A. V., & Myronchenko, S. I. (2010). Pro-inflammatory cytokines in the development of an experimental burn. Bulletin of the Higher Scientific-Educational Institution “Ukrainian Medical Dental Academy”, 10(4), 78-82.

[23] Zviahyntseva, T. V., Kryvoshapka, A. V., & Zhelnyn, E. V. (2014). The role of nitric oxide metabolites in the mechanisms of experimental burn development. Experimental and clinical medicine, 2(51), 5-9.
How to Cite
Mironov, Y. (2019). Histological picture in the skin of rats within a month after burn II-III degree against the background of the introduction for first 7 days 0.9% NaCl solution. Reports of Morphology, 25(4), 17-23. https://doi.org/https://doi.org/10.31393/morphology-journal-2019-25(4)-03