Electron microscopic changes in the skin of rats 1, 3, 7, 14, 21 and 30 days after thermal trauma on the background of the introduction for the first 7 days of 0.9% NaCl solution

  • O.Ye. Маievskyi Taras Shevchenko National University of Kyiv, Kyiv, Ukraine
  • Ye.V. Mironov National Pirogov Memorial Medical University, Vinnytsya, Ukraine
  • G.М. Galunko National Pirogov Memorial Medical University, Vinnytsya, Ukraine
  • N.P. Ocheretna National Pirogov Memorial Medical University, Vinnytsya, Ukraine
  • I.V. Gunas National Pirogov Memorial Medical University, Vinnytsya, Ukraine
Keywords: skin burns, electron microscopic changes, burn wound, epidermocytes, 0.9% NaCl solution.

Abstract

A burn wound occurs as a result of exposure to high-temperature skin or chemicals and is a serious injury with systemic effects. The problem of treatment of thermal trauma is urgent for modern medicine. Despite the fact that the overall mortality rate has dropped significantly in recent years, research about the development of new treatments and technologies for patients with skin burns is extremely important. The aim of the study is to research the features of electron microscopic changes in the skin of rats during the month after burns of II-III degree against the background of the introduction of the first 7 days of 0.9% NaCl solution. Studies were performed on 180 laboratory white rats-males weighing 155-160 g. In the course of the experiment, the animals were divided into 2 groups: 1 – rats without thermal injury, which were infused with 0.9% NaCl solution at a dose of 10 ml/kg; group 2 – rats which infused 0.9% NaCl solution at a dose of 10 ml/kg for 7 days after skin burns. Burning skin damage was caused by applying to the lateral surfaces of the trunk of rats for 10 seconds four copper plates, heated in water at a constant temperature of 100 C. The total area of skin lesion in rats was 21-23%. Shaving of the lateral surfaces of rats’ trunk, catheterization of veins, staging of skin burns, and decapitation of animals (after 1, 3, 7, 14, 21 and 30 days) were performed under intravenous propofol anesthesia (calculated at 60 mg/kg body weight). Preparations for electron microscopic examination were prepared according to the standard procedure. The data obtained were studied using a PEM-125K electron microscope. Electron microscopic studies of the skin of animals after thermal trauma under the conditions of application of 0.9% NaCl solution have found that in the early stages of the experiment – the stage of shock and early toxemia (1, 3, 7 days) compensatory and adaptive changes of its structural components are combined with signs of destructive disorders. In the epitheliocytes of the epidermis of the affected area of the skin is the destruction of nuclei and cytoplasm. Vascular disorders in the dermis are combined with changes in fibroblasts, an intercellular substance of fibrous connective tissue. In the late stages of the experiment – the stages of late toxemia and septicotoxemia (14, 21 and 30 days), there is a further development and deepening of destructive-dystrophic changes of all structural components of the affected skin, they become irreversible. The processes of granulation tissue formation, its transformation into connective tissue, as well as marginal epithelialization are slow. This morphological condition of the burn wound indicates the need for the use of corrective drugs to reduce destructive changes and the activation of regenerative processes in the area of skin lesions.

Downloads

Download data is not yet available.

References

[1] Abdullahi, A., Amini-Nik, S., & Jeschke, M. G. (2014). Animal models in burn research. Cell Mol. Life Sci., 71(17), 3241-3255. doi: 10.1007/s00018-014-1612-5
[2] Berezutsky, V. I. (2018). Modern approaches to therapy of burn shock. Pain, Аnesthesia and Intensive Care, 1(82), 35-40. doi: https://doi.org/10.25284/2519-2078.1(82).2018.121957
[3] Busuioc, C. J., Mogosanu, G. D., Popescu, F. C., Lascar, I., Parvanescu, H., & Mogoanta, L. (2013). Phases of the cutaneous angiogenesis process in experimental third-degree skin burns: histological and immunohistochemical study. Rom. J. Morphol. Embryol., 54(1), 163-171. PMID: 23529325
[4] Dobrelia, N. V., Boitsova, L. V., & Danova, I. V. (2015). Legal framework for conducting ethical expertise of preclinical studies of medicinal products using laboratory animals. Pharmacology and Drug Toxicology, 2, 95-100.
[5] Dries, D. J. & Marini, J. J. (2017). Management of Critical Burn Injuries: Recent Developments. Korean J. Crit. Care Med., 31(1), 9-21. doi: 10.4266/kjccm.2016.00969
[6] Galunko, G. M., & Gavryluk, A. O. (2014). Electron microscopic changes of the mucous membrane of the small intestine of rats in late stages of optic disease. World of Medicine and Biology, 4 (47), 97-101.
[7] Gunas, I., Dovgan, I., & Masur, O. (1997). Method of thermal burn trauma correction by means of cryoinfluence. Abstracts are presented in zusammen mit der Polish Anatomical Society with the participation of the Association des Anatomistes Verhandlungen der Anatomischen Gesellschaft, Olsztyn (р. 105). Jena – München : Der Urban & Fischer Verlag.
[8] Holdzon, M. A., Dolhykh, V. T., & Hyrsh, A. O. (2012). Violation of systemic hemodynamics, contractility and myocardial metabolism in severe thermal injury in the experiment and their correction. General Resuscitation, 8(3), 14-17.
[9] Horalskyi, L. P., Khomych, V. T., & Kononskyi, O. I. (2011). Fundamentals of histological technique and morphofunctional methods of research in normal and pathology. Zhytomyr: Polissya.
[10] Hur, J., Yang, H. T., Chun, W., Kim, J., Shin, S., Kang, H. L., & Kim, H. S. (2015). Inflammatory cytokines and their prognostic ability in cases of major burn injury. Ann. Lab. Med., 35(1), 105-110. doi: 10.3343/alm.2015.35.1.105
[11] Kallinen, O., Maisniemi, K., & Boheing, K. (2012). Multiple organ failure as cause of death in patients with severe burns. J. Burn Care Res, 33(2), 206-211. doi: 10.1097/BCR.0b013e3182331e73
[12] Klychnykova, E. V., Tazyna, E. V., Smyrnov, S. V., Spyrydonova, T. G., Zhyrkova, E. A., Borysov, V. S., & Godkov, M. A. (2015). Interrelation of biochemical indicators of oxidative stress, endogenous intoxication and regulation of vascular tone in patients with burn injury. Anesthesiology and Intensive Care, (1), 45-49.
[13] Kovalenko, O. M. (2014). Issues of infusion therapy for burn shock. Surgery of Ukraine, (2), 13-19.
[14] Kramar, S. B., Volkov, K. S., & Pyda, V. P. (2014). Submicroscopic changes of burn wound in experimental thermal trauma. Reports of Morphology, 20(1), 140-142.
[15] Masood, R. A., Wain, Z. N., Tariq, R., Ullah, M. A., & Bashir, I. (2016). Burn Cases, Their Management and Complications: A Review. International Current Pharmaceutical Journal, 5(12), 103-105. doi: 10.3329/icpj.v5i12.30411
[16] Matveev, S. B., Smyrnov, S. V., & Tazyna, E. V. (2013). The dynamics of endogenous intoxication in patients with extensive burns. Clinical Laboratory Diagnostics, 2, 10-12.
[17] Nebesna, Z. M., & Volkov, K. S. (2011). Ultrastructural changes of the components of the aerohematic barrier in the stage of burn disease toxemia. Ukrainian Morphological Almanac, 9(3), 191-192.
[18] Nebesna, Z. M., (2014). Submicroscopic changes of the components of the air-barrier barrier in the long term after the experimental thermal trauma. World of Medicine and Biology, 1(43), 138-142.
[19] Ocheretniuk, A. O., Nebesna, Z. M., Gunas, I. V., Yakovlieva, O. O., & Palamarchuk, O. V. (2013). Electron microscopic changes in the respiratory department of the lungs of rats after thermal injury of the skin under the conditions of application of NaCl solution. World of Medicine and Biology, 2(42), 38-41.
[20] Renneberg, B., Ripper, S., Schulze, J., Seehausen, A., Weiler, M., Wing, G., Hartmann, B., … Liedl, A. (2014). Quality of life and predictors of long-term outcome after severe burn injury. J. Behav. Med., 37(5), 967-976. doi: 10.1007/s10865-013-9541-6
[21] Rowan, M. P., Cancio, L. R., Elster, E. A., Burmeister, D. M., Rose, L. F., Natesan, S., Chan, R. K., … Chung, K. K. (2015). Burn wound healing and treatment: review and advancements. Crit. Care, 19, 1-12. doi: 10.1186/s13054-015-0961-2
Published
2020-02-28
How to Cite
МаievskyiO., Mironov, Y., Galunko, G., Ocheretna, N., & Gunas, I. (2020). Electron microscopic changes in the skin of rats 1, 3, 7, 14, 21 and 30 days after thermal trauma on the background of the introduction for the first 7 days of 0.9% NaCl solution. Reports of Morphology, 26(1), 5-13. https://doi.org/10.31393/morphology-journal-2020-26(1)-01

Most read articles by the same author(s)

1 2 > >>