Micro- and ultrastructural organization of the uterine wall of a white laboratory rat under conditions of 4-week action of Nalbuphine


  • Ya.T. Ivankiv Danylo Halytsky Lviv National Medical University, Lviv, Ukraine
Keywords: uterus, structure, opioids, experiment, reproductive health.

Abstract

The world community is facing a problem that threatens the entire population of the planet – reproductive health. This is a multifactorial problem that requires an integrated approach and effective solutions. One of the important factors that is very difficult to control and which in itself is a problem, and its prevalence can be called an epidemic without exaggeration, is drug addiction. The solution of these problems determined the purpose of our work – to establish what changes occur at the micro- and ultrastructural levels under the action of opioids on the uterus of white laboratory rats. The method of hematoxylin-eosin staining was used for histological examination, and the method of electron microscopy was used for the study at the cellular level. After 4 weeks of administration of Nalbuphine to white laboratory rats on histological sections, significant changes were observed in all layers of the uterus. The vessels of the myometrium were dilated, overflowing with erythrocytes, sometimes containing neutrophils and lymphocytes, perivasally marked the appearance of macrophages, in the cytoplasm of which was golden-brown pigment. The development of vacuolar dystrophy was also observed in the myometrium. There were necrotic changes and vacuolar dystrophy in the epithelium of the uterine mucosa, and epitheliocytes in which necrotic changes occurred exfoliated into the lumen of the uterus. The outer surface of the perimetrium was uneven due to necrotic changes of the monolayer squamous epithelium. Hyperplasia of the stratified squamous epithelium was observed in the ectocervix, in the areas of which a chaotic accumulation of low-differentiated epithelial cells was found. Electron microscopy revealed that a significant number of smooth myocytes lost their characteristic process shape, their nuclei were deformed and were in the stage of apoptosis, karyopyknosis and even karyorrhexis. The nucleoplasm contained almost no nucleoli, and heterochromatin is located on the periphery of the nucleus. The enlightened cytoplasm is filled with myofilaments, which are randomly arranged in the cell. Mitochondria acquired various shapes and sizes, mostly with destroyed cristae and areas of enlightenment. Swollen connective tissue layers between myocytes testify to circulatory disorders. The damage described in the experiment at all levels indicates the destructive effect of Nalbuphine, which causes irreversible changes that lead to significant violations of the structure and function of the uterus, which leads to the conclusion that excessive drug use has a direct negative impact on the reproductive system.

References

[1] Adams, K. T., Adams, T., Aldworth, J., Asman, K. J., Barnett, S. N., & Bishop, E. (2016). Results from the 2016 National Survey on Drug Use and Health: detailed tables. Center for Behavioral Health Statistics and Quality (CBHSQ), Substance Abuse and Mental Health Services Administration (SAMHSA), US Department of Health and Human Services (HHS), RTI International.

[2] Benningfield, M. M., Dietrich, M. S., Jones, H. E., Kaltenbach, K., Heil, S. H., Stine, S. M., ... Martin, P. R. (2012). Opioid dependence during pregnancy: relationships of anxiety and depression symptoms to treatment outcomes. Addiction, 107, 74-82. doi: 10.1111/j.1360-0443.2012.04041.x

[3] Dehghan, M., Jafarpour, M., & Mahmoudian, A. R. (2010). The effect of morphine administration on structure and ultrastructure of uterus in pregnant mice. Iranian Journal of Reproductive Medicine, 8(3), 111-118.

[4] El-Ghawet, H. A. (2015). Effects of tramadol on the reproductive function of wistar albino rats. Eur. J. Exp. Biol., 5, 56-64. Available online at www.pelagiaresearchlibrary.com

[5] Gorman, M. C., Orme, K. S., Nguyen, N. T., Kent III, E. J., & Caughey, A. B. (2014). Outcomes in pregnancies complicated by methamphetamine use. American Journal of Obstetrics and Gynecology, 211(4), 429-e1. doi: 10.1016/j.ajog.2014.06.005

[6] Government agency “Center for Mental Health and Monitoring of Drugs and Alcohol of the Ministry of Health of Ukraine” (2018). National report on the drug situation in Ukraine for 2018 (according to 2017).Kyiv. http://cmhmda.org.ua

[7] Horalskyi, L. P., Khomych, V. T., & Kononskyi, O. I. (2011). Fundamentals of histological technique and morphofunctional research methods in normal and pathology. Zhytomyr: Polissya.

[8] Ivankiv, Y. (2019). Structural organization of the links of hemomicrocircular channel of the rat`s uterus under the conditions of 6-week long administration of Nalbuphine. Proceeding of the Shevchenko Scientific Society. Medical Sciences, 55(1), 111-117. doi: https://doi.org/10.25040/ntsh2019.01.01

[9] Kayacan, N., Ertugrul, F., Arici, G., Karsli, B., Akar, M., & Erman, M. (2007). In vitro effects of opioids on pregnant uterine muscle. Advances in therapy, 24(2), 368-375. doi: 10.1007/BF02849906

[10] Mateshuk-Vatseba, L. R., Ivankiv, Ya. T., & Ivankiv, T. M. (2019). The influence of opioid on the microstructural organization of the Wall OT the uterus of the white laboratory rat. Clin. J. Obstet. Gynaecol., 2, 135-137. doi: dx.doi.org/10.29328/journal.cjog.1001036

[11] McHugh, R. K., DeVito, E. E., Dodd, D., Carroll, K. M., Potter, J. S., Greenfield, S. F., ... Weiss, R. D. (2013). Gender differences in a clinical trial for prescription opioid dependence. Journal of Substance Abuse Treatment, 45(1), 38-43. doi: 10.1016/j.jsat.2012.12.007

[12] NIDA (2020). Substance Use in Women. Retrieved from https://www.drugabuse.gov/publications/research-reports/substance-use-in-women/sum

[13] Pokotylo, V. Yu. (2017). Ultrastructural features of rat myocardium and its hemomicrocirculatory bed at the early stages of opioid intoxication. Bulletin of Problems of Biology and Medicine, 1(135), 304-309.

[14] Substance Abuse and Mental Health Services Administration (SAMHSA). (2011). Addressing the needs of women and girls: Developing core competencies for mental health and substance abuse service professionals. U.S. Department of Health and Human Services.

[15] Wizemann, T. M., & Pardue, M. L. (2001). Institute of Medicine (US) Committee on Understanding the Biology of Sex and Gender Differences. Exploring the biological contributions to human health: does sex matter. Washington (DC): National Academies Press (US).

[16] World Health Organization. Department of Mental Health, Substance Abuse, World Health Organization, International Narcotics Control Board, United Nations Office on Drugs, & Crime. (2009). Guidelines for the psychosocially assisted pharmacological treatment of opioid dependence. World Health Organization.

[17] Yoo, K. Y., Lee, J., Kim, H. S., & Jeong, S. W. (2001). The effects of opioids on isolated human pregnant uterine muscles. Anesthesia & Analgesia, 92(4), 1006-1009. doi: 10.1097/00000539-200104000-00037

[18] Yuzko, O. M. (2016). Reproductive health benefits: medical and social aspects. Health, (6), 3-6.
Published
2020-05-22
How to Cite
Ivankiv, Y. (2020). Micro- and ultrastructural organization of the uterine wall of a white laboratory rat under conditions of 4-week action of Nalbuphine. Reports of Morphology, 26(1), 32-36. https://doi.org/https://doi.org/10.31393/morphology-journal-2020-26(1)-05