Micro- and submicroscopic changes of the cerebellar cortex 21 days after modeling the burn

  • N.V. Ohinska I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
  • Z.M. Nebesna I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
  • I.B. Getmanyuk I. Horbachevsky Ternopil National Medical University, Ternopil, Ukraine
Keywords: cerebellar cortex, micro- and submicroscopic changes, neurons, thermal trauma.


The cerebellum is a complexly organized multifunctional component of the brain and a target in numerous lesions, so the study of its morphofunctional state in various pathological conditions and burns does not lose its relevance. The aim of our study was to establish the features of micro- and submicroscopic changes in the structural components of the cerebellar cortex after 21 days under conditions of experimental thermal trauma. The experimental study was simulated on white laboratory male rats. Grade III burns were applied under thiopental-sodium anesthesia with copper plates heated in boiled water to a temperature of 97-100°C. The size of the affected area was 18-20% of the epilated surface of the body of rats. Histological changes were studied for 21 days from the beginning of the experiment. Semi-thin sections for light microscopy were stained with methylene blue, for electron microscopy the obtained ultra-thin sections were contrasted with uranyl acetate and lead citrate according to the Reynolds method. With the help of micro- and submicroscopic studies, significant alternative changes of both neurons of all layers of the cerebellar cortex and parts of the microcirculatory tract of the organ were revealed. Neurons are disorganized, their shape is changed, there is low functional activity, due to a decrease in the area of the chromatophilic substance. Often there are cell-free areas in the ganglion layer, there is a shift of Purkinje cells deep into the granular layer, and grain cells are pushed sharply into the higher molecular layer. Thus, 21 days after the experimental thermal injury, destructive-degenerative changes of neurons of the cerebellar cortex, paravasal edema and disorders of transendothelial metabolism were established.


[1] Ashida, R., Cerminara, N. L., Brooks, J., & Apps, R. (2018). Principles of organization of the human cerebellum: macro- and microanatomy. Handbook of Clinical Neurology, 45-58. doi: 10.1016/b978-0-444-63956-1.00003-5
[2] Bostan, A. C., & Strick, P. L. (2018). The basal ganglia and the cerebellum: nodes in an integrated network. Nature Reviews Neuroscience, 19(6), 338-350. doi: 10.1038/s41583-018-0002-7
[3] Bratby, P., Sneyd, J., & Montgomery, J. (2016). Computational Architecture of the Granular Layer of Cerebellum-Like Structures. The Cerebellum, 16(1), 15-25. doi: 10.1007/s12311-016-0759-z
[4] Caligiore, D., Pezzulo, G., Baldassarre, G., Bostan, A. C., Strick, P. L., Doya, K., … Herreros, I. (2016). Consensus Paper: Towards a Systems-Level View of Cerebellar Function: the Interplay Between Cerebellum, Basal Ganglia, and Cortex. The Cerebellum, 16(1), 203-229. doi: 10.1007/s12311-016-0763-3
[5] Coombes, S. A., & Misra, G. (2016). Pain and motor processing in the human cerebellum. Pain, 157(1), 117-127. doi: 10.1097/j.pain.0000000000000337
[6] Delion, M., Dinomais, M., & Mercier, P. (2016). Arteries and Veins of the Cerebellum. The Cerebellum, 16(5-6), 880-912. doi:10.1007/s12311-016-0828-3
[7] Greenhalgh, D. G. (2019). Management of Burns. New England Journal of Medicine, 380(24), 2349-2359. doi: 10.1056/nejmra1807442
[8] Hicks, K. E., Huynh, M. N. Q., Jeschke, M., & Malic, C. (2019). Dermal regenerative matrix use in burn patients: A systematic review. Journal of Plastic, Reconstructive & Aesthetic Surgery, 72(11), 1741-1752. doi: 10.1016/j.bjps.2019.07.021
[9] Horalskyi, L. P., Khomych, V. T., & Kononskyi, O. I. (2015). Fundamentals of histological technique and morphofunctionalmethods of research in normal and pathology. Zhytomyr: Polissya. http://ir.znau.edu.ua/handle/123456789/3788
[10] Jeschke, M. G., van Baar, M. E., Choudhry, M. A., Chung, K. K., Gibran, N. S., & Logsetty, S. (2020). Burn injury. Nature Reviews Disease Primers, 6(1). doi: 10.1038/s41572-020-0145-5
[11] Kearney, L., Francis, E. C., & Clover, A. J. (2018). New technologies in global burn care – a review of recent advances. Int. J. Burns Trauma, 8(4), 77-87.
[12] Korytskyi, V. G., Nebesna, Z. M., & Kramar, S. B. (2019). Restructuring of vessels of the thyroid gland after experimental thermal injury along with application of minced substrate of freeze-dried xenograft. World of Medicine and Biology, 68(2), 183-187. doi: 10.26724/2079-8334-2019-2-68-183-187
[13] Mateshuk-Vatseba, L. R., & Bekesevych, A. M. (2015). Structural organization of rat cerebellar cortex under conditions of 6-week introduction of opioids. Сlinical Anatomy and Operative Surgery, 14(2), 69-71.
[14] Morozov, Yu. E., Dorosheva, Zh. V., Gornostaev, D. V., Koludarova, E. M., & Pigolkin, Yu. I. (2018). The morphological characteristic of the cerebellar cortex in the case of a burning injury. Forensic Medical Expertise, 4, 24-27. doi: 10.17116/sudmed201861424
[15] Rabellino, D., Densmore, M., Thеberge, J., McKinnon, M. C., & Lanius, R. A. (2018). The cerebellum after trauma: Resting-state functional connectivity of the cerebellum in posttraumatic stress disorder and its dissociative subtype. Human Brain Mapping, 39(8), 3354-3374. doi:10.1002/hbm.24081
[16] Stoodley, C. J., & Schmahmann, J. D. (2018). Functional topography of the human cerebellum. Handbook of Clinical Neurology, 59-70. doi: 10.1016/b978-0-444-63956-1.00004-7
[17] Tejiram, S., Romanowski, K. S., & Palmieri, T. L. (2019). Initial management of severe burn injury. Current Opinion in Critical Care, 25(6), 647-652. doi: 10.1097/mcc.0000000000000662
[18] Wallauer, M. M., Huf, F., Tortorelli, L. S., Rahmeier, F. L., Carvalho, F. B., Meurer, R. T., & da Cruz Fernandes, M. (2018). Morphological changes in the cerebellum as a result of ethanol treatment and cigarette smoke exposure: A study on astrogliosis, apoptosis and Purkinje cells. Neuroscience Letters, 672, 70-77. doi: 10.1016/j.neulet.2018.02.047
[19] Zhang, Q.-H., Hao, J.-W., Xiao-Jing, J., Guang-Lei, L., Zhou, M., & Yao, Y.-M. (2019). Long-lasting neurobehavioral alterations in burn-injured mice resembling post-traumatic stress disorder in humans. Experimental Neurology, 113084. doi:10.1016/j.expneurol.2019.113084
How to Cite
Ohinska, N., Nebesna, Z., & Getmanyuk, I. (2020). Micro- and submicroscopic changes of the cerebellar cortex 21 days after modeling the burn. Reports of Morphology, 26(3), 52-57. https://doi.org/10.31393/morphology-journal-2020-26(3)-07