Morphometric characteristics of distal airways of guinea pigs sensitized with ovalbumin

  • S.S. Popko Zaporizhzhia State Medical University, Zaporizhzhia, Ukraine
  • V.M. Evtushenko Zaporizhzhia State Medical University, Zaporizhzhia, Ukraine
Keywords: airways, allergic inflammation, Ovalbumin, guinea pig.

Abstract

The reaction of the structural components of small bronchi and terminal bronchioles is the urgent issue of morphology and medicine in general, as well as one of the insufficiently studied issue in the study of morphological changes in the airways with allergic inflammation in the chronobiological aspect. The purpose of this work is to study the morphometric parameters of distal airways of guinea pigs sensitized with ovalbumin. We have studied the lung of 48 guinea pigs, using histological, morphometric and statistical methods under conditions of experimental ovalbumin-induced allergic inflammation, simulated by three times subcutaneous sensitization and subsequent 8-day intranasal inhalation of ovalbumin. The thickness of mucosa, muscular layer and adventitial layer was determined to assess morphometric parameters of bronchioles and terminal bronchioles. There are reactive changes in morphometric parameters of bronchioles on the 23rd and 30th days of observation, such as the thinning of mucosa and on the 36th day such as muscular-fibrous hyperplasia, accompanied by the narrowing of the bronchioles lumen. There are the most significant confirmed statistically changes in the terminal bronchioles on the 36th day of the experiment such as the thickening of mucosa and adventitial layer, accompanied by muscular hyperplasia and edema of the connective tissue stroma. Thus, sensitization and allergization with ovalbumin of experimental animals cause morpho-functional changes in the structural elements of the wall of bronchioles and terminal bronchioles, which have a staged, mainly multidirectional character and correspond to the main morphological manifestations of allergic inflammation with maximal changes in the late period of its development (the 36th day of the experiment).

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References

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Published
2020-12-28
How to Cite
Popko, S., & Evtushenko, V. (2020). Morphometric characteristics of distal airways of guinea pigs sensitized with ovalbumin. Reports of Morphology, 26(4), 42-47. https://doi.org/10.31393/morphology-journal-2020-26(4)-07