Quantitative changes in the microstructure of the pancreas under the influence of sublethal dehydration, subsequent readaptation and correction

  • V.Yu. Kovchun Medical Institute of Sumy State University, Sumy, Ukraine
Keywords: pancreas, islets of Langerhans, acini, sublethal dehydration, thiazotic acid morpholinium salt.


Dehydration is a pathological condition caused by insufficient water intake and is accompanied by metabolic disorders that have significant consequences for human health and performance. The endocrine system takes part in a number of functions of the water exchange system, optimizing body fluid volume. In the literature, there is no systematic data on changes in the pancreas under conditions of various types of dehydration. This article is devoted to the study of structural changes in the pancreas in conditions of sublethal degrees of various types of dehydration, followed by readaptation and correction with a drug that has anti-ischemic, membrane-stabilizing, antioxidant, hepatoprotective and immunomodulatory properties that allow to normalize protein, carbohydrate and lipid metabolism. The purpose of the study was to research the features of changes in the endocrine and exocrine parenchyma of the pancreas by the method of morphometry of histological preparations under conditions of sublethal dehydration with subsequent readaptation and correction with thiazotic acid morpholinium salt. The study group consisted of 60 mature male rats, which were simulated sublethal degree of general, cellular and extracellular dehydration by the method of A.D.Soboleva, V.Z.Sikora, J.Ya.Bodnar. After reaching a severe degree of dehydration, part of the rats were transferred to a regular drinking diet, the second part received a corrector drug for 14 days. The animals were withdrawn from the experiment by decapitation under anesthesia on 24th, 44th and 104th days in accordance with the type of dehydration. After analyzing the results obtained, it was found that there were no significant differences in most morphometric parameters under conditions of exposure to sublethal dehydration in comparison with subsequent readaptation. Significant changes in the area of the islets of Langerhans and the area of nuclei of exocrinocytes were revealed under conditions of exposure to various types of sublethal dehydration in comparison with readaptation and correction; in all experimental groups. Other morphometric parameters of the pancreatic parenchyma had changes of varying statistical significance, which depended on the type of dehydration. It has been established that the use of the thiazotic acid morpholinium salt leads to partial restoration of the structural components of the pancreatic parenchyma in comparison with the period of readaptation.


[1] Ali, H., Al Dhaheri, A., Elmi, F., Ng, S., Zaghloul, S., Ohuma, E., & Qazaq, H. (2019). Water and Beverage Consumption among a Nationally Representative Sample of Children and Adolescents in the United Arab Emirates. Nutrients, 11(9), 2110. doi: 10.3390/nu11092110
[2] Barley, O., Chapman, D., & Abbiss, C. (2020). Reviewing the current methods of assessing hydration in athletes. Journal of the International Society of Sports Nutrition, 17(1). doi: 10.1186/s12970-020-00381-6
[3] Bruni, A., Bornstein, S., Linkermann, A., & Shapiro, A. (2018). Regulated Cell Death Seen through the Lens of Islet Transplantation. Cell Transplantation, 27(6), 890-901. doi: 10.1177/0963689718766323
[4] Davydova, L., Tkach, G., German, S., Bushtruk, A., & Maksymova, O. (2017). Особливості морфогенезу язика щурів при порушенні водно-електролітного балансу організму [Features of morphogenesis of rat tongue in violation of water-electrolyte balance of the organism]. Здобутки клінічної і експериментальної медицини – Achievements of Clinical and Experimental Medicine, 1(3). doi: 10.11603/1811-2471.2017.v1.i3.7956
[5] Davydova, L., Tkach, G., Tymoshenko, A., Moskalenko, A., Sikora, V., Kyptenko, L., Lyndin, M. … Suchonos, O. (2017). Anatomical and morphological aspects of papillae, epithelium, muscles, and glands of rats’ tongue: Light, scanning, and transmission electron microscopic study. Interventional Medicine and Applied Science, 9(3), 168-177. doi: 10.1556/1646.9.2017.21
[6] Cheuvront, S., Kenefick, R., Charkoudian, N., & Sawka, M. (2013). Physiologic basis for understanding quantitative dehydration assessment. The American Journal of Clinical Nutrition, 97(3), 455-462. doi: 10.3945/ajcn.112.044172
[7] Gamble, J., & McIver, M. (1928). Body fluid changes due to continued loss of the external secretion of the pancreas. Journal of Experimental Medicine, 48(6), 859-869. doi: 10.1084/jem.48.6.859
[8] James, L., Funnell, M., James, R., & Mears, S. (2019). Does Hypohydration Really Impair Endurance Performance? Methodological Considerations for Interpreting Hydration Research. Sports Medicine, 49(S2), 103-114. doi: 10.1007/s40279-019-01188-5
[9] Johnson, R., Lichtenstein, A., Anderson, C., Carson, J., Després, J., Hu, F. … Wylie-Rosett, J. (2018). Low-Calorie Sweetened Beverages and Cardiometabolic Health: A Science Advisory From the American Heart Association. Circulation, 138(9). doi: 10.1161/cir.0000000000000569
[10] Malisova, O., Athanasatou, A., Pepa, A., Husemann, M., Domnik, K., Braun, H. … Kapsokefalou, M. (2016). Water Intake and Hydration Indices in Healthy European Adults: The European Hydration Research Study (EHRS). Nutrients, 8(4), 204. doi: 10.3390/nu8040204
[11] Merkulov, G.A. (1969). Курс патологической техники [Pathological Technique Course]. М.: Медицина – M.: Medicine.
[12] Monaghan, T., Kavoussi, A., Agudelo, C., Rahman, S., Michelson, K., & Bliwise, D. et al. (2020). Nocturnal Urine Production in Women With Global Polyuria. International Neurourology Journal, 24(3), 270-277. doi: 10.5213/inj.2040166.083.
[13] Muñoz, C., Johnson, E., McKenzie, A., Guelinckx, I., Graverholt, G., & Casa, D. et al. (2015). Habitual total water intake and dimensions of mood in healthy young women. Appetite, 92, 81-86. doi: 10.1016/j.appet.2015.05.002
[14] Özen, A., Bibiloni, M., Bouzas, C., Pons, A., & Tur, J. (2018). Beverage Consumption among Adults in the Balearic Islands: Association with Total Water and Energy Intake. Nutrients, 10(9), 1149. doi: 10.3390/nu10091149
[15] Lugea, A., Gerloff, A., Su, H., Xu, Z., Go, A., & Hu, C. et al. (2017). The Combination of Alcohol and Cigarette Smoke Induces Endoplasmic Reticulum Stress and Cell Death in Pancreatic Acinar Cells. Gastroenterology, 153(6), 1674-1686. doi: 10.1053/j.gastro.2017.08.036
[16] Reid, I. (1994). Role of Nitric Oxide in the Regulation of Renin and Vasopressin Secretion. Frontiers in Neuroendocrinology, 15(4), 351-383. doi: 10.1006/frne.1994.1014
[17] Rybolovlev, Yu.R., & Rybolovlev, R.S. (1979). Дозирование веществ для млекопитающих по константе биологической активности [Dosing of substances for mammals according to the constant of biological activity]. Доклады АН СССР – Reports of the USSR Academy of Sciences.
[18] Sahin-Tóth, M., & Hegyi, P. (2017). Smoking and Drinking Synergize in Pancreatitis: Multiple Hits on Multiple Targets. Gastroenterology, 153(6), 1479-1481. doi: 10.1053/j.gastro.2017.10.031
[19] Savoie, F., Kenefick, R., Ely, B., Cheuvront, S., & Goulet, E. (2015). Effect of Hypohydration on Muscle Endurance, Strength, Anaerobic Power and Capacity and Vertical Jumping Ability: A Meta-Analysis. Sports Medicine, 45(8), 1207-1227. doi: 10.1007/s40279-015-0349-0
[20] Sharon, N., Chawla, R., Mueller, J., Vanderhooft, J., Whitehorn, L., Rosenthal, B. … Melton, D. (2019). A Peninsular Structure Coordinates Asynchronous Differentiation with Morphogenesis to Generate Pancreatic Islets. Cell, 176(4), 790-804.e13. doi: 10.1016/j.cell.2018.12.003
[21] Srinivasan, M.P., Bhopale, K.K., Caracheo, A.A., Amer, S.M., Khan, S., Kaphalia, L. … Kaphalia, B.S. (2020). Activation of AMP-activated protein kinase attenuates ethanol-induced ER/oxidative stress and lipid phenotype in human pancreatic acinar cells. Biochemical Pharmacology, 180, 114174. doi: 10.1016/j.bcp.2020.114174
[22] Scratcherd, T., Hutson, D., & Case, R.M. (1981). Ionic transport mechanisms underlying fluid secretion by the pancreas. Philosophical Transactions of the Royal Society B., 296(1080), 167-178. doi: 10.1098/rstb.1981.0180
[23] Tan, B., Philipp, M., Hill, S., Che Muhamed, A., & Mündel, T. (2020). Pain Across the Menstrual Cycle: Considerations of Hydration. Frontiers in Physiology, 11. doi: 10.3389/fphys.2020.585667
[24] Vieux, F., Maillot, M., Constant, F., & Drewnowski, A. (2017). Water and beverage consumption patterns among 4 to 13-year-old children in the United Kingdom. BMC Public Health, 17(1). doi: 10.1186/s12889-017-4400-y
[25] Villiger, M., Stoop, R., Vetsch, T., Hohenauer, E., Pini, M., Clarys, P. … Clijsen, R. (2017). Evaluation and review of body fluids saliva, sweat and tear compared to biochemical hydration assessment markers within blood and urine. European Journal of Clinical Nutrition, 72(1), 69-76. doi: 10.1038/ejcn.2017.136
[26] Zhang, J., Zhang, N., Liu, S., Du, S., He, H., & Ma, G. (2021). The comparison of water intake patterns and hydration biomarkers among young adults with different hydration statuses in Hebei, China. Nutrition & Metabolism, 18. doi: 10.1186/s12986-020-00531-2
[27] Zhang, J., Zhang, N., Wang, Y., Liang, S., Liu, S., Du, S. … Ma, G. (2020). Drinking patterns and hydration biomarkers among young adults with different levels of habitual total drinking fluids intake in Baoding, Hebei Province, China: a cross-sectional study. BMC Public Health, 20(1). doi: 10.1186/s12889-020-08558-z
[28] Zhang, J., Zhang, N., He, H.R., Cai, H., Yan, X.Y., Guo, X.H. … Ma, G. (2019). The total fluids intake, volume of urine and hydration status among young adults from Hebei Province in spring. Zhonghua Yu Fang Yi Xue Za Zhi, 53(4), 6. doi: 10.3760/cma.j.issn.0253-9624.2019.04.005
How to Cite
Kovchun, V. (2021). Quantitative changes in the microstructure of the pancreas under the influence of sublethal dehydration, subsequent readaptation and correction. Reports of Morphology, 27(2), 70-75. https://doi.org/10.31393/morphology-journal-2021-27(2)-10