OFFICIAL JOURNAL OF THE SCIENTIFIC SOCIETY OF
ANATOMISTS, HISTOLOGISTS, EMBRYOLOGISTS AND
TOPOGRAPHIC ANATOMISTS OF UKRAINE
ВІСНИК МОРФОЛОГІЇ
REPORTS OF MORPHOLOGY
DOI: 10.31393
ISSN 1818-1295
eISSN 2616-6194
Vol. 28, №1, 2022
Scientific peer-reviewed journal in the fields of normal and pathological anatomy, histology, cytology
and embryology, topographical anatomy and operative surgery, biomedical anthropology, ecology,
molecular biology, biology of development
Published since 1993
Periodicity: 4 times a year
Vinnytsya 2022
ORIGINAL ARTICLES
ВIСНИК МОРФОЛОГIЇ - REPORTS OF MORPHOLOGY
Founded by the "Scientific Society of Anatomists, Histologists, Embryologists, and Topographic
Anatomists of Ukraine" and National Pyrogov Memorial Medical University, Vinnytsya in 1993
Certificate of state registration КВ №9310 from 02.11.2004
Professional scientific publication of Ukraine in the field of medical sciences in specialties 221, 222, 228, 229
According to the list of professional scientific publications of Ukraine, approved by the order of the Ministry of Education and Science of Ukraine
No. 1188 of 24.09.2020
Professional scientific publication of Ukraine in the field of biological sciences in specialty 091
According to the list of professional scientific publications of Ukraine, approved by the order of the Ministry of Education and Science of Ukraine
No. 1471 of 26.11.2020
Chairman of the editorial board - Chaikovsky Yu.B. (Kyiv)
Vice-chairman of editorial board - Pivtorak V.I. (Vinnytsya), Kovalchuk O.I. (Kyiv)
Responsible editor - Gunas I.V. (Vinnytsya)
Secretary - Kaminska N.A. (Vinnytsya)
Editorial Board Members:
Berenshtein E.L. (Jerusalem), Byard R. (Adelaida), Dgebuadze М.А. (Tbilisi), Graeb C. (Hof),
Gulmen M.K. (Adana), Guminskyi Yu.Y. (Vinnytsya), Herashchenko S.B. (Ivano-Frankivsk),
Juenemann A.G.M. (Rostock), Kryvko Yu.Ya. (Lviv), Ocheredko O.M. (Vinnytsya), Rejdak R. (Lublin),
Sarafyniuk L.А. (Vinnytsya), Shepitko V.I. (Poltava), Shinkaruk-Dykovytska M.M. (Vinnytsya),
Stechenko L.O. (Kyiv), Wójcik Waldemar (Lublin)
Editorial council:
Appelhans O.L. (Odessa), Bulyk R.Ye. (Chernivtsi), Fedonyuk L.Ya. (Ternopil), Fomina L.V. (Vinnytsya),
Furman Yu.M. (Vinnytsya), Gerasymyuk I.Ye. (Ternopil), Golovatskyy A.S. (Uzhgorod), Kostylenko Yu.P.
(Poltava), Lutsyk O.D. (Lviv), Maievskyi O.Ye. (Kyiv), Mateshuk-Vatseba L.R. (Lviv), Mishalov V.D. (Kyiv),
Nebesna Z.M. (Ternopil), Olkhovskyy V.O. (Kharkiv), Piskun R.P. (Vinnytsya), Rudyk S.K. (Kyiv),
Sherstyuk O.O. (Poltava), Shevchuk Yu.G. (Vinnytsya), Sikora V.Z. (Sumy), Skybo G.G. (Kyiv),
Slobodian O.M. (Chernivtsi), Shkolnikov V.S. (Vinnytsya), Sokurenko L.M. (Kyiv), Tereshchenko V.P.
(Kyiv), Topka E.G. (Dnipro), Tverdokhlib I.V. (Dnipro), Yatsenko V.P. (Kyiv), Yeroshenko G.A. (Poltava)
Approved by the Academic Council of National Pyrogov Memorial Medical University, Vinnytsya, protocol
№5 from 27.01.2022.
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Address editors and publisher:
Pyrogov Str. 56,
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Tel.: +38 (0432) 553959
E-mail: nila@vnmu.edu.ua
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Translator - Gunas V.I.
Technical support - Levenchuk S.S., Parashuk O.I.
Scientific editing - editorship
The site of the magazine - https://morphology-journal.com
ORIGINAL ARTICLES
CONTENT
Zaiats L.M., Fedorchenko Yu.V. The role of neutrophilic granulocytes in the development of acute
lung injury in experimental diabetes mellitus ...................................................................................................... 5
Al-Omary Ala'a Osama Ahmad, Vadzyuk S. N., Shkolnikov V. S., Prokopenko S. V., Gunas I. V.
Discriminant models of possibilities occurrence and features of the course of different forms
of eczema in men depending on the characteristics of anthropometric indicators ............................................ 11
Maryenko N.I., Stepanenko O.Yu. Fractal analysis of anatomical structures linear contours: modified
Caliper method vs Box counting method........................................................................................................... 17
Pidvalna U. Ye., Beshley D. M., Mateshuk-Vatseba L. R. CT assessment of the height of the coronary
arteries orifice location and the height of the aortic sinuses in women with structural changes in the
coronary arteries ............................................................................................................................................... 27
Malachkova N.V., Mohammad Mashhour Mohammad Masa'deh Investigation of the effect of TNF-α
on damage to retinal pigment epithelial cells in age-related macular degeneration ........................................... 34
Oshurko A. P. Densitometric assessment in the justification of rehabilitation of patients with atrophy
of the bone tissue of the mandible, on the right side ......................................................................................... 42
Abdullaiev V. E. The results of morphological studies in women of reproductive age with hyperproliferative
diseases of the endometrium ............................................................................................................................ 48
Popko S. S. Morphological features of the respiratory part in guinea pigs lung in dynamics of experimental
allergic inflammatory process ........................................................................................................................... 54
Kondor Yu. Yu., Tykholaz V. O., Guminskyi Yu. Yо. Histological structure of intercellular fluid circulation
pathways .......................................................................................................................................................... 59
Usenko O. Yu., Sydiuk A. V., Sydiuk O. Ye., Klimas A. S., Savenko G. Yu., Teslia O. T. Morphometric
indicators for selection of dual endobronchial tube in thoracic anesthesiology.................................................. 64
REPORTS OF MORPHOLOGY
Official Journal of the Scientific Society of Anatomists,
Histologists, Embryologists and Topographic Anatomists
of Ukraine
© 2022 National Pirogov Memorial Medical University, Vinnytsya
journal homepage: https://morphology-journal.com
5
DOI: 10.31393/morphology-journal-2022-28(1)-01 Reports of Morphology, Vol. 28, №1, Pages 5-10
ISSN 1818-1295 eISSN 2616-6194
Introduction
Today, diabetes mellitus (DM) occupies one of the first
places in the structure of endocrine diseases [4, 18].
According to the scientific literature in recent years, there
has been a steady increase in the number of patients with
diabetes [1, 8, 22, 23, 27]. According to the WHO, by 2030
the number of patients with diabetes will reach 438 million
(6-8% of the adult population), with more than 90% are
The role of neutrophilic granulocytes in the development of acute
lung injury in experimental diabetes mellitus
Zaiats L.M., Fedorchenko Yu.V.
Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine
Diabetes mellitus takes one of the first places in the structure of endocrine diseases.
Among the complications of diabetes are sufficiently described nephro- and retinopathy,
neuropathy, damage to the cardiovascular system. However, changes in the respiratory
system and, in particular, the state of the endothelium of the pulmonary hemocapillaries
and the morphofunctional state of neutrophilic granulocytes remain poorly understood.
The aim of this study was to determine the role of neutrophilic granulocytes in the
pathogenesis of acute lung injury in experimental diabetes mellitus. The experiments
were performed on 88 white male Wistar rats weighing 170-210 g. The animals were
divided into three groups: 1 - intact (n=10); 2 - control (n=40); 3 - experimental (n=38)
with a model of diabetes mellitus, which was reproduced by intraperitoneal administration
of streptozotocin company "Sigma" (USA), diluted in 0.1 M citrate buffer with pH 4.5, at
a rate of 60 mg/kg body weight. An equivalent dose of 0.1 M citrate buffer solution with
a pH of 4.5 was intraperitoneally administered to the control group of animals. Pulmonary
tissue collection for electron microscopic examination was performed under thiopental
anesthesia 14, 28, 42, 70 days after streptozotocin administration. Pieces of lung
tissue were fixed in 2.5% glutaraldehyde solution, followed by fixation in 1% osmium
tetroxide solution. After dehydration, the material was poured into epon-araldite. Sections
obtained on an ultramicrotome "Tesla BS-490" were studied in an electron microscope
"PEM-125K". It was found that in the early stages of diabetes mellitus (14-28 days)
there is a violation of the rheological properties of blood, as evidenced by erythrocyte
aggregates, excessive accumulation of neutrophils, their adhesion and aggregation in
the hemocapillaries of the alveolar wall. With the extension of the experiment (42-70
days) there is a progressive violation of the ultrastructural organization of
hemocapillaries of the alveolar wall and pronounced changes in the rheological
properties of blood. Erythrocyte sludges and leuco-platelet aggregates are determined
in the lumen of microvessels. Increased permeability of hemocapillaries of the alveolar
wall leads to the emigration of neutrophilic granulocytes into the interstitium and the
lumen of the alveoli with the development of interstitial and intraalveolar edema. Thus,
streptozotocin-induced diabetes is accompanied by the development of acute lung
damage in the pathogenesis of which the leading role belongs to neutrophilic
granulocytes. The nature and severity of changes in the lungs depends on the duration
of exposure to hyperglycemia.
Keywords: neutrophilic granulocytes, hemocapillaries, lungs, diabetes mellitus.
ARTICLE INFO
Received: 3 November 2021
Accepted: 15 December 2021
UDC: 616.24+616.092.9+616.379-
008.64
CORRESPONDING AUTHOR
e-mail: patfisiology@ifnmu.edu.ua
Zaiats L.M.
CONFLICT OF INTEREST
The authors have no conflicts of interest
to declare.
FUNDING
Not applicable.
people with type 2 diabetes [7, 15]. The severity of this
problem is determined not only by the significant prevalence
of diabetes in the population, but also the rapid
development of complications that lead to reduced quality
of life, early disability and premature death [10, 21, 24].
According to literature sources, among the complications
of diabetes today are sufficiently described nephro- and