OFFICIAL JOURNAL OF THE SCIENTIFIC SOCIETY OF

ANATOMISTS, HISTOLOGISTS, EMBRYOLOGISTS AND

TOPOGRAPHIC ANATOMISTS OF UKRAINE

ВІСНИК МОРФОЛОГІЇ

REPORTS OF MORPHOLOGY

DOI: 10.31393

ISSN 1818-1295

eISSN 2616-6194

Vol. 28, №1, 2022

Scientific peer-reviewed journal in the fields of normal and pathological anatomy, histology, cytology

and embryology, topographical anatomy and operative surgery, biomedical anthropology, ecology,

molecular biology, biology of development

Published since 1993

Periodicity: 4 times a year

Vinnytsya  2022

ORIGINAL ARTICLES

ВIСНИК МОРФОЛОГIЇ - REPORTS OF MORPHOLOGY

Founded by the "Scientific Society of Anatomists, Histologists, Embryologists, and Topographic

Anatomists of Ukraine" and National Pyrogov Memorial Medical University, Vinnytsya in 1993

Certificate of state registration КВ №9310 from 02.11.2004

Professional scientific publication of Ukraine in the field of medical sciences in specialties 221, 222, 228, 229

According to the list of professional scientific publications of Ukraine, approved by the order of the Ministry of Education and Science of Ukraine

No. 1188 of 24.09.2020

Professional scientific publication of Ukraine in the field of biological sciences in specialty 091

According to the list of professional scientific publications of Ukraine, approved by the order of the Ministry of Education and Science of Ukraine

No. 1471 of 26.11.2020

Chairman of the editorial board - Chaikovsky Yu.B. (Kyiv)

Vice-chairman of editorial board - Pivtorak V.I. (Vinnytsya), Kovalchuk O.I. (Kyiv)

Responsible editor - Gunas I.V. (Vinnytsya)

Secretary - Kaminska N.A. (Vinnytsya)

Editorial Board Members:

Berenshtein E.L. (Jerusalem), Byard R. (Adelaida), Dgebuadze М.А. (Tbilisi), Graeb C. (Hof),

Gulmen M.K. (Adana), Guminskyi Yu.Y. (Vinnytsya), Herashchenko S.B. (Ivano-Frankivsk),

Juenemann A.G.M. (Rostock), Kryvko Yu.Ya. (Lviv), Ocheredko O.M. (Vinnytsya), Rejdak R. (Lublin),

Sarafyniuk L.А. (Vinnytsya), Shepitko V.I. (Poltava), Shinkaruk-Dykovytska M.M. (Vinnytsya),

Stechenko L.O. (Kyiv), Wójcik Waldemar (Lublin)

Editorial council:

Appelhans O.L. (Odessa), Bulyk R.Ye. (Chernivtsi), Fedonyuk L.Ya. (Ternopil), Fomina L.V. (Vinnytsya),

Furman Yu.M. (Vinnytsya), Gerasymyuk I.Ye. (Ternopil), Golovatskyy A.S. (Uzhgorod), Kostylenko Yu.P.

(Poltava), Lutsyk O.D. (Lviv), Maievskyi O.Ye. (Kyiv), Mateshuk-Vatseba L.R. (Lviv), Mishalov V.D. (Kyiv),

Nebesna Z.M. (Ternopil), Olkhovskyy V.O. (Kharkiv), Piskun R.P. (Vinnytsya), Rudyk S.K. (Kyiv),

Sherstyuk O.O. (Poltava), Shevchuk Yu.G. (Vinnytsya), Sikora V.Z. (Sumy), Skybo G.G. (Kyiv),

Slobodian O.M. (Chernivtsi), Shkolnikov V.S. (Vinnytsya), Sokurenko L.M. (Kyiv), Tereshchenko V.P.

(Kyiv), Topka E.G. (Dnipro), Tverdokhlib I.V. (Dnipro), Yatsenko V.P. (Kyiv), Yeroshenko G.A. (Poltava)

Approved by the Academic Council of National Pyrogov Memorial Medical University, Vinnytsya, protocol

№5 from 27.01.2022.

Indexation: CrossRef, Index Copernicus, Google Schoolar Metrics, National Library of Ukraine

Vernadsky

Address editors and publisher:

Pyrogov Str. 56,

Vinnytsya, Ukraine - 21018

Tel.: +38 (0432) 553959

E-mail: nila@vnmu.edu.ua

Computer page-proofs - Klopotovska L.O.

Translator - Gunas V.I.

Technical support - Levenchuk S.S., Parashuk O.I.

Scientific editing - editorship

The site of the magazine - https://morphology-journal.com

ORIGINAL ARTICLES

CONTENT

Zaiats L.M., Fedorchenko Yu.V. The role of neutrophilic granulocytes in the development of acute

lung injury in experimental diabetes mellitus ...................................................................................................... 5

Al-Omary Ala'a Osama Ahmad, Vadzyuk S. N., Shkolnikov V. S., Prokopenko S. V., Gunas I. V.

Discriminant models of possibilities occurrence and features of the course of different forms

of eczema in men depending on the characteristics of anthropometric indicators ............................................ 11

Maryenko N.I., Stepanenko O.Yu. Fractal analysis of anatomical structures linear contours: modified

Caliper method vs Box counting method........................................................................................................... 17

Pidvalna U. Ye., Beshley D. M., Mateshuk-Vatseba L. R. CT assessment of the height of the coronary

arteries orifice location and the height of the aortic sinuses in women with structural changes in the

coronary arteries ............................................................................................................................................... 27

Malachkova N.V., Mohammad Mashhour Mohammad Masa'deh Investigation of the effect of TNF-α

on damage to retinal pigment epithelial cells in age-related macular degeneration ........................................... 34

Oshurko A. P. Densitometric assessment in the justification of rehabilitation of patients with atrophy

of the bone tissue of the mandible, on the right side ......................................................................................... 42

Abdullaiev V. E. The results of morphological studies in women of reproductive age with hyperproliferative

diseases of the endometrium ............................................................................................................................ 48

Popko S. S. Morphological features of the respiratory part in guinea pigs lung in dynamics of experimental

allergic inflammatory process ........................................................................................................................... 54

Kondor Yu. Yu., Tykholaz V. O., Guminskyi Yu. Yо. Histological structure of intercellular fluid circulation

pathways .......................................................................................................................................................... 59

Usenko O. Yu., Sydiuk A. V., Sydiuk O. Ye., Klimas A. S., Savenko G. Yu., Teslia O. T. Morphometric

indicators for selection of dual endobronchial tube in thoracic anesthesiology.................................................. 64

REPORTS OF MORPHOLOGY

Official Journal of the Scientific Society of Anatomists,

Histologists, Embryologists and Topographic Anatomists

of Ukraine

journal homepage: https://morphology-journal.com

DOI: 10.31393/morphology-journal-2022-28(1)-01 Reports of Morphology, Vol. 28, №1, Pages 5-10

ISSN 1818-1295 eISSN 2616-6194

Introduction

Today, diabetes mellitus (DM) occupies one of the first

places in the structure of endocrine diseases [4, 18].

According to the scientific literature in recent years, there

has been a steady increase in the number of patients with

diabetes [1, 8, 22, 23, 27]. According to the WHO, by 2030

the number of patients with diabetes will reach 438 million

(6-8% of the adult population), with more than 90% are

The role of neutrophilic granulocytes in the development of acute

lung injury in experimental diabetes mellitus

Zaiats L.M., Fedorchenko Yu.V.

Ivano-Frankivsk National Medical University, Ivano-Frankivsk, Ukraine

Diabetes mellitus takes one of the first places in the structure of endocrine diseases.

Among the complications of diabetes are sufficiently described nephro- and retinopathy,

neuropathy, damage to the cardiovascular system. However, changes in the respiratory

system and, in particular, the state of the endothelium of the pulmonary hemocapillaries

and the morphofunctional state of neutrophilic granulocytes remain poorly understood.

The aim of this study was to determine the role of neutrophilic granulocytes in the

pathogenesis of acute lung injury in experimental diabetes mellitus. The experiments

were performed on 88 white male Wistar rats weighing 170-210 g. The animals were

divided into three groups: 1 - intact (n=10); 2 - control (n=40); 3 - experimental (n=38)

with a model of diabetes mellitus, which was reproduced by intraperitoneal administration

of streptozotocin company "Sigma" (USA), diluted in 0.1 M citrate buffer with pH 4.5, at

a rate of 60 mg/kg body weight. An equivalent dose of 0.1 M citrate buffer solution with

a pH of 4.5 was intraperitoneally administered to the control group of animals. Pulmonary

tissue collection for electron microscopic examination was performed under thiopental

anesthesia 14, 28, 42, 70 days after streptozotocin administration. Pieces of lung

tissue were fixed in 2.5% glutaraldehyde solution, followed by fixation in 1% osmium

tetroxide solution. After dehydration, the material was poured into epon-araldite. Sections

obtained on an ultramicrotome "Tesla BS-490" were studied in an electron microscope

"PEM-125K". It was found that in the early stages of diabetes mellitus (14-28 days)

there is a violation of the rheological properties of blood, as evidenced by erythrocyte

aggregates, excessive accumulation of neutrophils, their adhesion and aggregation in

the hemocapillaries of the alveolar wall. With the extension of the experiment (42-70

days) there is a progressive violation of the ultrastructural organization of

hemocapillaries of the alveolar wall and pronounced changes in the rheological

properties of blood. Erythrocyte sludges and leuco-platelet aggregates are determined

in the lumen of microvessels. Increased permeability of hemocapillaries of the alveolar

wall leads to the emigration of neutrophilic granulocytes into the interstitium and the

lumen of the alveoli with the development of interstitial and intraalveolar edema. Thus,

streptozotocin-induced diabetes is accompanied by the development of acute lung

damage in the pathogenesis of which the leading role belongs to neutrophilic

granulocytes. The nature and severity of changes in the lungs depends on the duration

of exposure to hyperglycemia.

Keywords: neutrophilic granulocytes, hemocapillaries, lungs, diabetes mellitus.

ARTICLE INFO

Received: 3 November 2021

Accepted: 15 December 2021

UDC: 616.24+616.092.9+616.379-

008.64

CORRESPONDING AUTHOR

e-mail: patfisiology@ifnmu.edu.ua

Zaiats L.M.

CONFLICT OF INTEREST

The authors have no conflicts of interest

to declare.

FUNDING

Not applicable.

people with type 2 diabetes [7, 15]. The severity of this

problem is determined not only by the significant prevalence

of diabetes in the population, but also the rapid

development of complications that lead to reduced quality

of life, early disability and premature death [10, 21, 24].

According to literature sources, among the complications

of diabetes today are sufficiently described nephro- and