Modeling of viral-bacterial infections against antibiotic-induced intestinal dysbiosis

  • V.V. Bobyr Bogomolets National Medical University, Kyiv, Ukraine
  • L.О. Stechenko Bogomolets National Medical University, Kyiv, Ukraine
  • V.P. Shyrobokov Bogomolets National Medical University, Kyiv, Ukraine
  • О.І. Cryvosheyeva Bogomolets National Medical University, Kyiv, Ukraine
  • О.А. Nazarchuk National Pirogov Memorial Medical University, Vinnytsya, Ukraine
  • V.А. Ponyatovskyi Bogomolets National Medical University, Kyiv, Ukraine
  • S.М. Chuhrai Bogomolets National Medical University, Kyiv, Ukraine
Keywords: antimicrobials, bacteria, viruses, dysbiosis, intestinal microbiocenosis, intestinal infections.

Abstract

The study of the role of viral-bacterial associations in the etiology of acute intestinal infections is considered new to medical microbiology. The purpose of the study was to determine the effect of viral-bacterial associations on the manifestation of virulence of pathogens and the degree of development of structural-morphological disorders of the internal organs in animals with antibiotic-induced dysbiosis. In a study of 210 white laboratory mice, BALB/c lines formed dysbiosis using antibacterial agents (ampicillin, gentamicin, metronidazole) followed by simulation of the experimental infection. To simulate salmonella infection, mice were infected with a clinical strain of Salmonella typhimurium intraperitoneally. Similarly, the animals were infected with Coxsackie B3 test culture virus (dose 106 TCD50). The sensitivity of mice to Coxsackie B and salmonella viruses was examined for mortality and disease characteristics. The animals were removed from the experiment 24 h after infection, electronically microscopically studied structural and morphological changes of the internal organs were performed. There was no statistically significant difference in morbidity (23.33-26.66 %) and mortality (16.66-20.0 %) of mice infected with Coxsackie virus with dysbiotic disorders and preserved microflora. Dysbiotic conditions have been shown to lead to associated viral-bacterial infections in animals and, accordingly, an increase in the incidence of disease and death in experimental animals. Against the background of disturbance of the composition of the normal intestine microbiota in viral bacterial infections, pronounced degenerative changes in the internal organs of animals were established, with signs of generalization. Electrogram data showed the appearance of activation of immunocompetent cells of the body in viral-bacterial infection in animals with impaired intestine microbiocenosis.

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References

[1] Ahmed, S. M., Hall, A. J., Robinson, A. E., Verhoef, L., Premkumar, P., Parashar, U. D., ... Lopman, B. A. (2014). Global prevalence of norovirus in cases of gastroenteritis: a systematic review and meta-analysis. The Lancet infectious diseases, 14(8), 725-730. doi: 10.1016/S1473-3099(14)70767-4
[2] Andes, D. R., & Lepak, A. J. (2017). In vivo infection models in the pre-clinical pharmacokinetic/pharmacodynamic evaluation of antimicrobial agents. Current opinion in pharmacology, 36, 94-99. doi: 10.1016/j.coph.2017.09.004
[3] Bobyr, V. V., Ponyatovskiy, V. A., Djugikowa, E. M., & Shyrobokov, V. P. (2015). Modeling of dysbiotic disorders with laborat ory animals. Biomedical and Biosocial Anthropology, 24, 230-233.
[4] Chkhenkeli, V. A., Anisimova, A. V., Romanova, E. D., Kalinovich, A. Ye., & Promtov, M. V. (2014). Experimental assessment of the efficiency of the “Trametin” at experimental salmonellosis of laboratory and agricultural animals. Bulletin of the Eastern Siberian scientific center of the siberian branch of the Russian academy of medical sciences, 6(100), 80-83.
[5] E’ssel’, A. E., Panteleeva, L. G., & Myasnenko, A. M. (1978). Viral bacterial associations. Publishing House Rostovsk. University.
[6] Gorelov, A. V., & Usenko, D. V. (2008). Rotavirus infection in children. Current Pediatrics, 7(6), 78-84.
[7] Gyssens, I. C. (2019). Animal models for research in human infectious diseases. CMI editorial policy. Clinical microbiology and infection: the official publication of the European Society of Clinical Microbiology and Infectious Diseases, 25(6), 649. https://doi.org/10.1016/j.cmi.2019.04.010
[8] Kim, B. H., Kim, T. H., & Lee, M. K. (2016). A Clinico-Epidemiological Comparison Study of Pediatric Acute Viral Gastroenteritis at a Tertiary Care Hospital. Annals of Clinical Microbiology, 19(2), 33-38. https://doi.org/10.5145/ACM.2016.19.2.33
[9] Kotloff, K. L. (2017). The burden and etiology of diarrheal illness in developing countries. Pediatric Clinics, 64(4), 799-814.
[10] Kulieva, Z. M. (2015). Characteristcs of mixed intestinal infections of early age with emergency conditions. Russian Medical Journal, 21(4), 21-23.
[11] Marsh, E. K., & May, R. C. (2012). Caenorhabditis elegans, a model organism for investigating immunity. Appl. Environ. Microbiol., 78(7), 2075-2081. doi: 10.1128/AEM.07486-11
[12] Osborne, N., Avey, M. T., Anestidou, L., Ritskes‐Hoitinga, M., & Griffin, G. (2018). Improving animal research reporting standards. EMBO reports, 19(5), e46069. doi: 10.15252/embr.201846069
[13] Park, J. O., Jeon, J. S., & Kim, J. K. (2019). Epidemiologic Trends of Diarrhea-causing Virus Infection Analyzed by Multiplex Reverse Transcription PCR in Cheonan, Korea, 2010-2018. Microbiology and Biotechnology Letters, 47(2), 317-322. https://doi.org/10.4014/mbl.1811.11007
[14] Pollok-Waksmańska, W., Słowiaczek, K., & Wijas, D. (2019). Salmonellosis among children hospitalized in Pediatric Hospital in Bielsko-Biała, Poland, in the years 2014-2015. Medycyna doświadczalna i mikrobiologia, 71, 5-12. doi: 10.32394/mdm.71.01
[15] Rhoades, N., Barr, T., Hendrickson, S., Prongay, K., Haertel, A., Gill, L., ... & Messaoudi, I. (2019). Maturation of the infant rhesus macaque gut microbiome and its role in the development of diarrheal disease. Genome biology, 20(1), 1-16. https://doi.org/10.1186/s13059-019-1789-x
[16] Study of mixed infections in vivo. Retrieved from http://techpharm.ru/epidemiology1-120
[17] Swearengen, J. R. (2018). Choosing the right animal model for infectious disease research. Animal models and experimental medicine, 1(2), 100-108. https://doi.org/10.1002/ame2.12020
[18] Tokar, R. G., Zakstel’skaya, L. Ya., Shendorovich, S. F. (1971). The results of co-infection of tissue culture with influenza virus A2 and Staphylococcus. Journal of microbiology epidemiology immunobiology, 6, 100-103.
[19] World Health Organization (WHO) (2017). Diarrhea [Electronic source]. WHO Bulleting. Retrieved from http://www.who.int/ru/news-room/fact-sheets/detail/diarrhoeal-disease
Published
2019-06-26
How to Cite
Bobyr, V., Stechenko, L., Shyrobokov, V., CryvosheyevaО., NazarchukО., Ponyatovskyi, V., & Chuhrai, S. (2019). Modeling of viral-bacterial infections against antibiotic-induced intestinal dysbiosis. Reports of Morphology, 25(2), 78-84. https://doi.org/10.31393/morphology-journal-2019-25(2)-10